User:Klare Lazor/Notebook/Chem-496-001/2011/08/31: Difference between revisions
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==Description== | ==Description== | ||
Protocol: | |||
Materials needed included Chloroauric Acin (HAuCl4), bovine serum albumen (BSA), and zein protein. BSA(0.0015-0.015mM) and HAuCl4(0.25-10mM) were used to create 10mL aqueous mixtures. Each mixture was transferred to a screw-capped glass bottle. the mixtures were kept in a water thermostat bath a 40, 60, or 80±1˚C for 6 hours. The solutions changed from colorless to pink-purple and finally purple. The samples were then removed from the bath and left to cool overnight. Last, the were purified with distilled water two times in order to remove any excess BSA, using a centrifuge at 10,000-14,000rpm for five minutes. | |||
The samples were placed in different temperature baths to observe how temperature affects the synthesis of the gold nanoparticles. In addition, in order to determine the absorbance caused by surface plasmo resonance, UV-visible spectra were taken of each sample using an UV Spectrophotometer with a wavelength range of 200-900nm. Time dependent scans were also taken to collect data on the growth kinetics of AU nao0particles. Other data was collected using a pH meter, SEM, TEM, X-ray diffraction and AFM. | |||
==Data== | ==Data== | ||
Revision as of 14:22, 4 September 2011
 Biomaterials Design Lab
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ObjectiveThe purpose this experiment is to determine the ideal pH for the synthesis of Au(0) nanoparticles. For this experiment, Au(0) nanoparticles will be created by reducing HAuCl4 with bovine serum albumen (BSA) in an aqueous solution with a pH of 7.55. DescriptionProtocol: Materials needed included Chloroauric Acin (HAuCl4), bovine serum albumen (BSA), and zein protein. BSA(0.0015-0.015mM) and HAuCl4(0.25-10mM) were used to create 10mL aqueous mixtures. Each mixture was transferred to a screw-capped glass bottle. the mixtures were kept in a water thermostat bath a 40, 60, or 80±1˚C for 6 hours. The solutions changed from colorless to pink-purple and finally purple. The samples were then removed from the bath and left to cool overnight. Last, the were purified with distilled water two times in order to remove any excess BSA, using a centrifuge at 10,000-14,000rpm for five minutes. The samples were placed in different temperature baths to observe how temperature affects the synthesis of the gold nanoparticles. In addition, in order to determine the absorbance caused by surface plasmo resonance, UV-visible spectra were taken of each sample using an UV Spectrophotometer with a wavelength range of 200-900nm. Time dependent scans were also taken to collect data on the growth kinetics of AU nao0particles. Other data was collected using a pH meter, SEM, TEM, X-ray diffraction and AFM. Data
NotesThis area is for any observations or conclusions that you would like to note.
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