User:Klare Lazor/Notebook/Chem-496-001/2011/09/14: Difference between revisions
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==Data== | ==Data== | ||
[[Image:Asept14.png]] | [[Image:Asept14.png]] | ||
Image 1: Is a linear fit of concentration vs. absorbance for the wavelength of 495nm for the corrected values without the bradford reagent. | |||
[[Image:Bsept14.png]] | |||
Image 2: Absorbance of just the protein blank in water no bradford reagent. These values will be able to determine the concentration of the unknown protein. | |||
Revision as of 19:03, 27 September 2011
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ObjectiveTo determine the concentration of an unknown protein, a Bradford Assay was used. DescriptionTo determine the concentration of an unknown protein, a Bradford Assay was performed. Materials needed included Bradford Reagent, bovine serum albumen (BSA), and protein (MBP). Standard solutions of BSA (1mL each) in water of 10, 8, 6, 4, 2, and 1ug/mL were prepared. First, 3mL of a 10ug/mL stock solution was created. From this stock solution the standards, as well as the sample of unknown protein, were made. The sample of the unknown protein should be 1000 times too concentrated. Furthermore, a blank for the Bradford Reagent and the protein were prepared. A UV-visible spectra was taken of each solution with a wavelength range of 200-800nm. DataImage 1: Is a linear fit of concentration vs. absorbance for the wavelength of 495nm for the corrected values without the bradford reagent.
Image 2: Absorbance of just the protein blank in water no bradford reagent. These values will be able to determine the concentration of the unknown protein.
NotesObservations:
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