User:Klare Lazor/Notebook/Chem-496-001/2011/09/14

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Objective

To determine the concentration of an unknown protein, a Bradford Assay was used.

Description

To determine the concentration of an unknown protein, a Bradford Assay was performed. Materials needed included Bradford Reagent, bovine serum albumen (BSA), and protein (MBP). Standard solutions of BSA (1mL each) in water of 10, 8, 6, 4, 2, and 1ug/mL were prepared. First, 3mL of a 10ug/mL stock solution was created. From this stock solution the standards, as well as the sample of unknown protein, were made. The sample of the unknown protein should be 1000 times too concentrated. Furthermore, a blank for the Bradford Reagent and the protein were prepared. A UV-visible spectra was taken of each solution with a wavelength range of 200-800nm.

Data

  • Add data and results here...

Notes

Observations:

  • It was observed that the color of the Bradford Reagent upon addition to the protein was blue, and slowly, turned back to red when the concentration of the protein decreased.


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