User:Klare Lazor/Notebook/Chem-496-001/2011/10/04: Difference between revisions

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*Using the spectrum produced by the concentrations, the molar absorptivity was determine.
*Using the spectrum produced by the concentrations, the molar absorptivity was determine.
*The real concentration of the estimated solutions were determined by using the equation of the line of the from the previous protien lab, y=.218x.
*The real concentration of the estimated solutions were determined by using the equation of the line of the from the previous protien lab, y=.218x.
**0.2ug/ml--> .053211 ug/ml
**2ug/ml-->.53211ug/ml
**10ug/ml-->2.665ug/ml
**100.0ug/ml--> 26.05ug/ml


==Data==
==Data==

Revision as of 06:34, 11 October 2011

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Objective

Determine the fluorescence of MBP as a function of concentration. As well as, figure out the concentration of MBP based on its absorbance spectrum, and the sensitivity of the new fluorescence instrument. To determine the sensitivity of the fluorescence instrument, you will need to make a standard solution of Ru(bpy)(phen) in acteonitrile. Note: To get an accurate determination of fluorescence intensity, you don't measure just the intensity at the emission peak. You measure the integrated intensity of the entire emission.

Description

  • 0.2, 2.0, 10.0, 100.0 (original solution), and 1000.0 ug/mL estimated concentrations of MBP were used.
  • Using the spectrum produced by the concentrations, the molar absorptivity was determine.
  • The real concentration of the estimated solutions were determined by using the equation of the line of the from the previous protien lab, y=.218x.
    • 0.2ug/ml--> .053211 ug/ml
    • 2ug/ml-->.53211ug/ml
    • 10ug/ml-->2.665ug/ml
    • 100.0ug/ml--> 26.05ug/ml

Data

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


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