User:Klare Lazor/Notebook/Chem-496-001/2011/11/15: Difference between revisions
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**24.228 grams of Tris and made to a volume of 1 Liter | **24.228 grams of Tris and made to a volume of 1 Liter | ||
* MW Tris--> 121.14 g/mol MW NaCl--> 58.4g/mol | * MW Tris--> 121.14 g/mol MW NaCl--> 58.4g/mol | ||
* all were calibrated to a ph of 9 using 3M HCl | |||
* the smaller flask were filtered for contaminants from tris and nacl | |||
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*they were than sonacated for 30 seconds and not for 30secounds. This process was repeated three times for each test tube of cells. | *they were than sonacated for 30 seconds and not for 30secounds. This process was repeated three times for each test tube of cells. | ||
* In principle, the high-frequency is generated electronically and the mechanical energy is transmitted to the sample via a metal probe that oscillates with high frequency. The probe is placed into the cell-containing sample and the high-frequency oscillation causes a localized low pressure region resulting in cavitation and impaction, ultimately breaking open the cells. Although the basic technology was developed over 50 years ago, newer systems permit cell disruption in smaller samples (including multiple samples under 200 µL in microplate wells) and with an increased ability to control ultrasonication parameters (wikipedia.com). | * In principle, the high-frequency is generated electronically and the mechanical energy is transmitted to the sample via a metal probe that oscillates with high frequency. The probe is placed into the cell-containing sample and the high-frequency oscillation causes a localized low pressure region resulting in cavitation and impaction, ultimately breaking open the cells. Although the basic technology was developed over 50 years ago, newer systems permit cell disruption in smaller samples (including multiple samples under 200 µL in microplate wells) and with an increased ability to control ultrasonication parameters (wikipedia.com). | ||
* Then tubes were weighed and then each brought to the same weight using water. common weight was roughly ~117 grams. | * Then tubes were weighed and then each brought to the same weight using water. common weight was roughly ~117 grams. And, placed into a centrifuge. | ||
* the tubes were centrifuged for 1 hour at 10000 RPM | |||
==Data== | ==Data== |
Revision as of 13:55, 15 November 2011
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Objective
Descriptiongreen test tubes......1:2 ratio of gold to BSA with a volume of 10mL
Also today we will make
Data
NotesThis area is for any observations or conclusions that you would like to note.
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