User:Klare Lazor/Notebook/Chem-496-001/2011/11/15: Difference between revisions
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* all were calibrated to a ph of 9 using 3M HCl | * all were calibrated to a ph of 9 using 3M HCl | ||
* the smaller flask were filtered for contaminants from tris and nacl | * the smaller flask were filtered for contaminants from tris and nacl | ||
'''[[PROTEIN EXTRACTION]]''' | '''[[PROTEIN EXTRACTION]]''' | ||
'''Sonacation of previously made cells''' | '''Sonacation of previously made cells''' | ||
*Cells that were centrifuged down and transferred to tubes, and then frozen to later thaw to aid in process of lysing. freeze/thaw has been shown to effectively release recombinant proteins located in the cytoplasm of bacteria and is recommended for the lysis of mammalian cells in some protocols. | *Cells that were centrifuged down and transferred to tubes, and then frozen to later thaw to aid in process of lysing. freeze/thaw has been shown to effectively release recombinant proteins located in the cytoplasm of bacteria and is recommended for the lysis of mammalian cells in some protocols. |
Revision as of 10:59, 16 November 2011
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Objective
Descriptiongreen test tubes......1:2 ratio of gold to BSA with a volume of 10mL
Also today we will make
Sonacation of previously made cells
DataNotesThis area is for any observations or conclusions that you would like to note.
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