User:Klare Lazor/Notebook/Chem-496-001/2011/11/15: Difference between revisions
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'''green test tubes......1:2 ratio of gold to BSA with a volume of 10mL''' | '''green test tubes......1:2 ratio of gold to BSA with a volume of 10mL''' | ||
*test tube A | *test tube A (Sent off to lab for analysis labeled GA) | ||
** 250µL of gold 4.17mM | ** 250µL of gold 4.17mM | ||
**500µL of BSA 30µM | **500µL of BSA 30µM | ||
** | **9250µL of H20 | ||
*test tube B | *test tube B (Sent off to lab for anlysis labeled GB) | ||
** 500µL of gold 4.17mM | ** 500µL of gold 4.17mM | ||
**1000µL of BSA 30µM | **1000µL of BSA 30µM | ||
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'''Red test tubes......1:1 ratio of gold to BSA with a volume of 10mL''' | '''Red test tubes......1:1 ratio of gold to BSA with a volume of 10mL''' | ||
*test tube A | *test tube A (Sent off to lab for analysis labeled RA) | ||
** 500µL of gold 4.17mM | ** 500µL of gold 4.17mM | ||
**500µL of BSA 30µM | **500µL of BSA 30µM | ||
** | **9000µL of H20 | ||
*test tube B | *test tube B (Sent off to lab for analysis labeled RB) | ||
**1000µL of gold 4.17mM | **1000µL of gold 4.17mM | ||
**1000µL of BSA 30µM | **1000µL of BSA 30µM | ||
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* the smaller flask were filtered for contaminants from tris and nacl | * the smaller flask were filtered for contaminants from tris and nacl | ||
'''[[PROTEIN EXTRACTION]]''' | |||
'''Sonacation of previously made cells''' | '''Sonacation of previously made cells''' | ||
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==Data== | ==Data== | ||
1:1 RATIO OF GOLD TO BSA RESULTS | |||
[[Image:Screen_Shot_2011-11-15_at_3.49.32_PM.png]] | [[Image:Screen_Shot_2011-11-15_at_3.49.32_PM.png]] | ||
1:2 RATIO OF GOLD TO BSA RESULTS | |||
* After two hours in the oven at 80 degrees Celsius nothing happened | |||
* After twenty-four hours the solution turned purple, NO FIBERS !! | |||
==Notes== | ==Notes== |
Revision as of 11:30, 16 November 2011
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Objective
Descriptiongreen test tubes......1:2 ratio of gold to BSA with a volume of 10mL
Also today we will make
Sonacation of previously made cells
Data1:1 RATIO OF GOLD TO BSA RESULTS
NotesThis area is for any observations or conclusions that you would like to note.
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