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Objective
- make purple solution
- make purple fibers
Description
green test tubes......1:2 ratio of gold to BSA with a volume of 10mL
- test tube A
- 250µL of gold 4.17mM
- 500µL of BSA 30µM
- 900µL of H20
- test tube B
- 500µL of gold 4.17mM
- 1000µL of BSA 30µM
- 8500µL of H20
- don't take out trying to make solution
Red test tubes......1:1 ratio of gold to BSA with a volume of 10mL
- test tube A
- 500µL of gold 4.17mM
- 500µL of BSA 30µM
- 900µL of H20
- test tube B
- 1000µL of gold 4.17mM
- 1000µL of BSA 30µM
- 8000µL of H20
- leave in for 50minutes and take out for 10 min
'''Note:''' Both concentrations are the same, but doubled from previous solutions or experiments.
Also today we will make
- 1 L 50mM Tris + 1M NaCl pH 9 Filter
- 6.05 grams of Tris + 58.4grams of Nacl and made to a volume of 1 Liter
- 1 L 50mM Tris pH 9 Filter
- 6.05 grams of Tris and made to a volume of 1 Liter
- 4 L 50mM Tris pH 9 (2x)
- 24.228 grams of Tris and made to a volume of 1 Liter
- MW Tris--> 121.14 g/mol MW NaCl--> 58.4g/mol
Sonacation of previously made cells
- Cells that were centrifuged down and transferred to tubes were sonacated for 30 seconds and not for 30secounds. This process was repeated three times for each test tube of cells.
- In principle, the high-frequency is generated electronically and the mechanical energy is transmitted to the sample via a metal probe that oscillates with high frequency. The probe is placed into the cell-containing sample and the high-frequency oscillation causes a localized low pressure region resulting in cavitation and impaction, ultimately breaking open the cells. Although the basic technology was developed over 50 years ago, newer systems permit cell disruption in smaller samples (including multiple samples under 200 µL in microplate wells) and with an increased ability to control ultrasonication parameters (wikipedia.com).
Data
- Add data and results here...
Notes
This area is for any observations or conclusions that you would like to note.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
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Biomaterials Design Lab
