# User:Klare Lazor/Notebook/Chem-496-001/2012/02/01

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## Objective

• synthesize AUNP at a concentration of 60 HAUCl4 to 1 BSA
• prepare stock solutions

## Description

• Stock Solutions
• HAuCl4 in 50mL solution
• 2.7mM= 0.0027mols/L x 0.05 L = 1.35E-4 mols HAuCl4
• 1.35E-4 mols HAuCl4 x 337.79 mols = 0.0458789 grams of HAuCl4
• BSA in 50mL solutions
• 1.5µM=0.000015mols/L x 0.05L = 7.5 E-7 mols
• 7.5 E-7 mols x 66,000 g/mol = 0.0495 grams of BSA

• BSA/AuNP Preparation of 60:1 based off stocks above
• In oven for four hours

Calculations for dilutions of 60:1 in 10 mL

• (0.906ml HAuCl4)(0.0027mols/L)= (10mL)(x)
• x=0.000245mols/L of HAuCl4 in 10mL of water total volume
• (60)/(1)=(0.000245)/(x)
• x=0.000004 mols/L of BSA
• (x)(.000015mols/L)=(10mL)/(0.000004 mols/L)
• x= 2.666 mL of BSA in 10mL of water total volume

• So,
• 2666 µL of HAuCl4
• 906 µL of BSA
• 6428 µL of H20

Check on ratio of concentration= (0.000009mols/L)/ (0.00000015mols/LBSA) = 60/1

## Data

• nothing, bsa stock was to dilute to produce any nano particles

## Notes

• No nano particles were made due to lack of HAuCl4.