User:Klare Lazor/Notebook/Chem-496-001/2012/02/06: Difference between revisions

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*So,
*So,
**2666 µL of HAuCl4
**2666 µL of bsa
**906 µL of BSA
**906 µL of HAuCl4
**6428 µL of H20
**6428 µL of H20


Revision as of 13:24, 26 March 2012

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Objective

  • remake solutions to determine what is going wrong

Description

  • Calculations for dilutions of 60:1 in 10 mL
  • Stock Solutions
    • HAuCl4 in 50mL solution
      • 2.7mM= 0.0027mols/L x 0.05 L = 1.35E-4 mols HAuCl4
      • 1.35E-4 mols HAuCl4 x 337.79 mols = 0.0458789 grams of HAuCl4
    • BSA in 50mL solutions
      • 1.57µM=0.000015mols/L x 0.05L = 7.5 E-7 mols
      • 7.5 E-7 mols x 66,000 g/mol = 0.0495 grams of BSA


  • BSA/AuNP Preparation of 60:1 based off stocks above
    • In oven for four hours


Calculations for dilutions of 60:1 in 10 mL

  • (0.906ml HAuCl4)(0.0027mols/L)= (10mL)(x)
    • x=0.000245mols/L of HAuCl4 in 10mL of water total volume
  • (60)/(1)=(0.000245)/(x)
    • x=0.000004 mols/L of BSA
  • (x)(.000015mols/L)=(10mL)/(0.000004 mols/L)
    • x= 2.666 mL of BSA in 10mL of water total volume


  • So,
    • 2666 µL of bsa
    • 906 µL of HAuCl4
    • 6428 µL of H20

Check on ratio of concentration= (0.000009mols/L)/ (0.00000015mols/LBSA) = 60/1

Data

  • finally realized bsa stock solution was to dilute remade solutions today with the correct
  • purple solution resulted

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


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