User:Klare Lazor/Notebook/Chem-496-001/2012/02/14: Difference between revisions

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****(2.776mL)(15µM)=(x)(3.182mL [which is the mL of BSA and Buffer total for the synthesis])
****(2.776mL)(15µM)=(x)(3.182mL [which is the mL of BSA and Buffer total for the synthesis])
****x= 13µM for the synthesis solution of dye to bsa
****x= 13µM for the synthesis solution of dye to bsa
'''****13µM(30/1030) =  .381 µM of dye in the dye+BSA solution'''
****'''13µM(30/1030) =  .381 µM of dye in the dye+BSA solution'''


NOTE: We will only make a concentration curve with the buffer and dye today
NOTE: We will only make a concentration curve with the buffer and dye today

Revision as of 12:38, 15 February 2012

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

  • Construct Concentration Curve
  • Test Fluorescence of BSA and Dye

Description

Concentration Curve

  • Make a curve from a blank of buffer and water. Range of concentrations of dye up to 50µM
    • CALCULATION STOCK OF 50µM OF DYE in BUFFER and WATER
      • 515.3g/mol * 50E-6mols/L * 1L/1000 x 50mL = 0.001 grams of dye in 50mL Actual for both stocks 0.0015grams
    • CALCULATIONS FOR CONCENTRATION CURVE DYE + BUFFER FIRST
      • 50E-6mols/L * x = 10E-6 mols/L * 0.001L
        • Add 200µL of stock to 800µL of buffer
      • 50E-6mols/L *x = 5E-6 mols/L * 0.001 L
        • Add 100µL of stock to 900µL of buffer
      • 50E-6mols/L * x = 1E-6mols/L * 0.001 L
        • Add 20µL stock to 980 µL of buffer
      • 50E-6mols/L * x = 2E-6mols/L * .001L
  • REAL CONCENTRATIONS NEED TO BE CALCULATED FROM THE USE OF 0.0015 grams for the stock
    • TEMPORARY ESTIMATED CONCENTRATIONS
      • 1µM, 2µM, 5µM, and 10µM
      • BSA +DYE .... 30µL Dye+BSA and 1000µL of Buffer phosphate
      • To determine the amount of dye in Dye+BSA
        • (2.776mL)(15µM)=(x)(3.182mL [which is the mL of BSA and Buffer total for the synthesis])
        • x= 13µM for the synthesis solution of dye to bsa
        • 13µM(30/1030) = .381 µM of dye in the dye+BSA solution

NOTE: We will only make a concentration curve with the buffer and dye today

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


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