User:Klare Lazor/Notebook/Chem-496-001/2012/02/28: Difference between revisions
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'''Remake of nano particles + dye calculations''' | '''Remake of nano particles + dye calculations''' | ||
*note two different solutions of nano particles were used to make this solution | |||
**10:1 ratio Buffer: Acid has to be taken into consideration because we do not want the acid to affect the buffer and change the pH and not deprotonate the cysteine | |||
*** y= buffer x= gold/bsa | |||
*** 0.05 m/L Buffer * xL = 10 ( 0.000276 m/L * yL] | |||
***x+Y = 0.003L | |||
*** y = 0.003L-x | |||
*** 0.05M/L (x) = 10 (0.000276mols/L x (0.003L-x)) | |||
**** x= 1.5693 E-4 L, so buffer is 0.1569mL | |||
****y=0.003L-1.569347E-4L, so 2.8432mL of au/bsa | |||
**To determine how much dye | |||
***BSA mols=0.004mM(2.843mL)= 1.137E-8 mols Au moles= 0.276mM (2.8342mL)= 7.8469E-7 moles | |||
***BSA Mols * 66,000g/mol= 7.505E-4 grams of BSA x 0.06329 mass ratio of cysteins to bsa= 4.75 E-5 grams cysteine | |||
*** 4.75E-5 g of cysteine / 424.06 g/ mole cysteine= 1.12E-8 moles of Cysteine | |||
*** 1.12E-8 moles of cysteine x 4mol dye/1mol of cysteine = 4.4808 E-8 mols of dye (MW of dye) = 2.309E-3 grams of dyes | |||
*Stock solution of dye | |||
**2.309E-3 * 10mL = 0.2309 g/L | |||
** .2309 g/L * xL = 2.309E-5 g | |||
** x= 1E-4L = 100µL | |||
'''What contained in new solution... just multiplied solution by 4 to get larger volume..''' | |||
* This time | |||
** buffer 0.6276mL | |||
** Au/BSA 11.3724 mL | |||
** Dye 9.236E-5 g | |||
** Total vol 12 mL | |||
*** solution was seen to be fluorescing more than last time. the solution was tinted slight orange/purple. | |||
* Last time | |||
** buffer 0.1569mL | |||
** Au/ Bsa 2.8431 mL | |||
** Dye 2.309E-5 g | |||
** Total vol 3 mL | |||
==Data== | ==Data== |
Revision as of 11:31, 21 February 2012
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Objective
DescriptionConcentration Curve
Remake of nano particles + dye calculations
Data
NotesThis area is for any observations or conclusions that you would like to note.
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