User:Klare Lazor/Notebook/Chem-496-001/2012/03/13: Difference between revisions

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==Notes==
==Notes==
* nothing happened with the bsa+dye and HAuCl4 because by accident it was made in a phosphate buffer and the pH did not allow the AU3+ to be reduced to Au0, so we will attempt again with batch 3 of bsa+day and HAucl4.




Revision as of 10:48, 7 March 2012

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Objective

  • added HAuCl4 to bsa+dye to see what happened in oven
  • test uv vis and fluorescence of batch 2 nanoparticle + dye

Description

BSA+DYE with added HAuCl4 : doing this to see if nano particles will form in presence of HAuCl4

Calculations

  • presently in dye+BSA there is 13µM of BSA
    • (2mL)(13µM)=(10mL)(x)
      • x= 0.000003M of BSA in BSA +DYE diluted to 10mL
        • x/(0.000003M BSA)= 60/1 x= 0.000156 M of HAuCl4
    • (10mL)(0.00156M HAuCl4)=(x)(0.00276M HAuCl4)
      • x= 0.565ml of gold

Volumes

  • 565µL of gold
  • 2000µL of bsa+gold
  • 7435µL of H20

Data

Notes

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


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