User:Klare Lazor/Notebook/Chem-496-001/2012/03/27: Difference between revisions

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==Objective==
==Description==
*made gold nano particles was successful, concluded has to be in a volume of 10ml something throws it off when made in bigger quantity
*TODAY DIALYSIS OF GOLD NANOPARTICLES IN DIFFERENT VOLUMES
*dialysis of gold nanopartilces and fluorescence before dialysis
*ADD BSA CONCENTRATION TO BSA CONCENTRATION CURVE


==Description==Dialysis
''Dialysis''
*Membrane
*Membrane
**Spectra/PorBiotech Cellulose Ester (CE) Dialysis Membrane MWCO: 100,000
**Spectra/PorBiotech Cellulose Ester (CE) Dialysis Membrane MWCO: 100,000
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==Data==
==Data==
* Add data and results here...
[[Image:AUNPs_before_dialysis_batch_1_and_2.png]]
 
Figure 1: image of absorbance of AuNPs before dialysis for batch 1 and batch 2, after dialysis uv spectra will be taken again to obtain the concentration of bsa bound to the nano particles.


==Notes==
==Notes==

Revision as of 11:50, 27 March 2012

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Description

  • TODAY DIALYSIS OF GOLD NANOPARTICLES IN DIFFERENT VOLUMES
  • ADD BSA CONCENTRATION TO BSA CONCENTRATION CURVE

Dialysis

  • Membrane
    • Spectra/PorBiotech Cellulose Ester (CE) Dialysis Membrane MWCO: 100,000
    • 100 kD
    • wet in 0.05% sodium azide
  • 1
    • about 1,000 ml water bath
    • 5 ml AuNPs b1
  • 2
    • 100 ml water bath
    • 3.5ml AUNP b2

Data

Figure 1: image of absorbance of AuNPs before dialysis for batch 1 and batch 2, after dialysis uv spectra will be taken again to obtain the concentration of bsa bound to the nano particles.

Notes

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