User:Klare Lazor/Notebook/Chem-496-001/2012/09/19: Difference between revisions
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General Protocol 1(klare and Liz): | General Protocol 1(klare and Liz): | ||
#Allow the glutaraldehyde PVOH film to completely air dry | #Allow the glutaraldehyde PVOH film to completely air dry | ||
#Prepare a solution of 100mL H<sub>2</sub>O, 2mL concentrated H<sub>2</sub>SO<sub>4</sub>, and 20g Na<sub>2</sub>SO<sub>4</sub> in a 150mL beaker. Place stir bar in beaker and a Buchner funnel top over the stir bar | #Prepare a solution of 100mL H<sub>2</sub>O, 2mL concentrated H<sub>2</sub>SO<sub>4</sub>, and 20g Na<sub>2</sub>SO<sub>4</sub> in a 150mL beaker. Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar | ||
#Heat the prepared solution to 70°C(on hot plate turn temperature to 160°C) | #Heat the prepared solution to 70°C(on hot plate turn temperature to 160°C) | ||
#Place film in solution for 1 hour.(Make sure the temperature remains at 70°C) | #Place film in solution for 1 hour.(Make sure the temperature remains at 70°C) | ||
#Prepare 100mL 1% aqueous HCl solution. Heat solution to 70°C | #Prepare 100mL 1% aqueous HCl solution. Heat solution to 70°C. (ie: 1ml of HCL in 100 ml) | ||
#Remove film from the solution and rinse with deionized H<sub>2</sub>O | #Remove film from the solution and rinse with deionized H<sub>2</sub>O | ||
#Place stir bar in 1% HCl solution and a Buchner funnel top over of the stir bar. Add the PVOH film to the solution | #Place stir bar in 1% HCl solution and a Buchner funnel top over of the stir bar. Add the PVOH film to the solution |
Revision as of 06:11, 19 September 2012
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Objective
DescriptionGeneral Protocol 1(klare and Liz):
General Protocol 2 (Noah):
Glutaraldehyde Prep
Data
NotesThis area is for any observations or conclusions that you would like to note.
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