User:Klare Lazor/Notebook/Chem-496-001/2012/09/19

Objective

1. Crosslink the prepared glutaraldehyde film
2. Run DSC on 8 films
3. Run FTIR
4. Repeat the crosslink of glutaraldehyde film

Description

General Protocol 1(klare and Liz):

1. Allow the glutaraldehyde PVOH film to completely air dry
2. Prepare a solution of 100mL H₂O, 2mL concentrated H₂SO₄, and 20g Na₂SO₄ in a 150mL beaker. Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar
3. Heat the prepared solution to 70°C(on hot plate turn temperature to 160°C)
4. Place film in solution for 1 hour.(Make sure the temperature remains at 70°C) Must be under fumehood because of glutaraldehyde
5. Prepare 100mL 1% aqueous HCl solution. Heat solution to 70°C. (ie: 1ml of HCL in 100 ml)
6. Remove film from the solution and rinse with deionized H₂O
7. Place stir bar in 1% HCl solution and a Buchner funnel top over of the stir bar. Add the PVOH film to the solution
8. Allow film to remain in 1% HCl solution for 30 minutes.(Be sure to keep a constant 70°C)
9. Prepare 0.2M sodium bicarbonate solution
10. After 30 minutes, remove film from 1% HCl solution and place in 0.2M sodium bicarbonate solution for ~15minutes
11. Remove film from dilute sodium bicarbonate solution with tweezers. Rinse with deionized H₂O and dry film on paper towel(dabbing gently).
12. Weigh PVOH film. Record mass= .8059g

General Protocol 2 (Noah):

1. Repeat Glutaraldehyde film prep
2. make 2nd glutaradehyde film film, except and replace 10% PVOH with PDMS
3. Prepare 1 L glutaraldehyde solutions
4. prepare 500ml 20ppm SO42- as CuSO4
5. prepare 500ml 20ppm SO42- as Na2SO4
6. dry clays

Glutaraldehyde Prep

• 0.5g PVOH, 5ml H2O => dissolve
• add 1ml Glutaraldehyde in fume hood
• 0.5132 g PVOH, 5ml h2o + 1ml glutaraldehyde
• 0.4055g PVOH, 0.1015 PDMS, 5ml h2o
• 200 g Na2So4, 1L H2O stirred to dissolution + 20ml H2 So4
• MW CuSO4 * 5 H20= 249.67 g/mole 20 ppm in 500ml H2O MW= 18 g/mol (500g of h20/18g/mol=27.7moles)
  • 27.7moles h2O* ((20moles cuso4*5H2O)/1,000,000 moles H2O) = 5.55 E -4 moles Cuso4*5molesH20
  • 5.55E-4moles CuSo4*5H2O * 249.68 g/mol= 0.1387g CuSo4*5H2O
  • 5.55E-4moles Na2SO4 * 142.04g/mol=0.078 (20ppm)

Data

• Add data and results here...

Notes

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