User:Klare Lazor/Notebook/Chem-496-001/2012/09/19

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Current revision (12:11, 7 November 2012) (view source)
 
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==Objective==
==Objective==
#Crosslink the prepared glutaraldehyde film
#Crosslink the prepared glutaraldehyde film
-
#Run DSC on 8 films
+
#Repeat the crosslink of glutaraldehyde film
-
#Run FTIR
+
==Description==
==Description==
General Protocol 1(klare and Liz):
General Protocol 1(klare and Liz):
#Allow the glutaraldehyde PVOH film to completely air dry
#Allow the glutaraldehyde PVOH film to completely air dry
-
#Prepare a solution of 100mL H<sub>2</sub>O, 2mL concentrated H<sub>2</sub>SO<sub>4</sub>, and 20g Na<sub>2</sub>SO<sub>4</sub> in a 150mL beaker. Place stir bar in beaker and a Buchner funnel top over the stir bar  
+
#Prepare a solution of 100mL H<sub>2</sub>O, 2mL concentrated H<sub>2</sub>SO<sub>4</sub>, and 20g Na<sub>2</sub>SO<sub>4</sub> in a 150mL beaker. Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar  
#Heat the prepared solution to 70°C(on hot plate turn temperature to 160°C)
#Heat the prepared solution to 70°C(on hot plate turn temperature to 160°C)
-
#Place film in solution for 1 hour.(Make sure the temperature remains at 70°C)
+
#Place film in solution for 1 hour.(Make sure the temperature remains at 70°C) '''Must be under fumehood because of glutaraldehyde'''
-
#Prepare 100mL 1% aqueous HCl solution. Heat solution to 70°C
+
#Prepare 100mL 1% aqueous HCl solution. Heat solution to 70°C. (ie: 1ml of HCL in 100 ml)
#Remove film from the solution and rinse with deionized H<sub>2</sub>O
#Remove film from the solution and rinse with deionized H<sub>2</sub>O
#Place stir bar in 1% HCl solution and a Buchner funnel top over of the stir bar. Add the PVOH film to the solution
#Place stir bar in 1% HCl solution and a Buchner funnel top over of the stir bar. Add the PVOH film to the solution
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#After 30 minutes, remove film from 1% HCl solution and place in 0.2M sodium bicarbonate solution for ~15minutes
#After 30 minutes, remove film from 1% HCl solution and place in 0.2M sodium bicarbonate solution for ~15minutes
#Remove film from dilute sodium bicarbonate solution with tweezers. Rinse with deionized H<sub>2</sub>O and dry film on paper towel(dabbing gently).
#Remove film from dilute sodium bicarbonate solution with tweezers. Rinse with deionized H<sub>2</sub>O and dry film on paper towel(dabbing gently).
-
#Weigh PVOH film. Record mass
+
#Weigh PVOH film. Record mass= .8059g
General Protocol 2 (Noah):
General Protocol 2 (Noah):
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# prepare 500ml 20ppm SO42- as Na2SO4
# prepare 500ml 20ppm SO42- as Na2SO4
#dry clays
#dry clays
 +
 +
'''Glutaraldehyde Prep and CuSO4*5H20 20ppm'''
 +
* 0.5g PVOH, 5ml H2O => dissolve
 +
* add 1ml Glutaraldehyde in fume hood
 +
*0.5132 g PVOH, 5ml h2o + 1ml glutaraldehyde
 +
*0.4055g PVOH, 0.1015 PDMS, 5ml h2o
 +
<s>*200 g Na2So4, 1L H2O stirred to dissolution + 20ml H2 So4 </s>
 +
<s>*MW CuSO4 * 5 H20= 249.67 g/mole 20 ppm in 500ml H2O MW= 18 g/mol  (500g of h20/18g/mol=27.7moles) </s>
 +
<s>**27.7moles h2O* ((20moles cuso4*5H2O)/1,000,000 moles H2O) = 5.55 E -4 moles Cuso4*5molesH20 </s>
 +
<s>**5.55E-4moles CuSo4*5H2O  *  249.68 g/mol= 0.1387g CuSo4*5H2O </s>
 +
<s>**5.55E-4moles Na2SO4 * 142.04g/mol=0.078 (20ppm) </s>
 +
<s>*we want 500ml of 20ppm = 20mg/l  CuSO4</s>
 +
<s>** 277.4mg/l*x=.5*20mg/l</s>
 +
<s>** x=36.05ml in total 100ml solution</s>
 +
<s>* we want 500ml of 20ppm= 20mg/l  Na2SO4</s>
 +
<s>** 156.8 mg/l x = .5(20mg/L)v
 +
<s>** x= 63.78ml in total 100ml solution</s>
==Data==
==Data==

Current revision

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Objective

  1. Crosslink the prepared glutaraldehyde film
  2. Repeat the crosslink of glutaraldehyde film

Description

General Protocol 1(klare and Liz):

  1. Allow the glutaraldehyde PVOH film to completely air dry
  2. Prepare a solution of 100mL H2O, 2mL concentrated H2SO4, and 20g Na2SO4 in a 150mL beaker. Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar
  3. Heat the prepared solution to 70°C(on hot plate turn temperature to 160°C)
  4. Place film in solution for 1 hour.(Make sure the temperature remains at 70°C) Must be under fumehood because of glutaraldehyde
  5. Prepare 100mL 1% aqueous HCl solution. Heat solution to 70°C. (ie: 1ml of HCL in 100 ml)
  6. Remove film from the solution and rinse with deionized H2O
  7. Place stir bar in 1% HCl solution and a Buchner funnel top over of the stir bar. Add the PVOH film to the solution
  8. Allow film to remain in 1% HCl solution for 30 minutes.(Be sure to keep a constant 70°C)
  9. Prepare 0.2M sodium bicarbonate solution
  10. After 30 minutes, remove film from 1% HCl solution and place in 0.2M sodium bicarbonate solution for ~15minutes
  11. Remove film from dilute sodium bicarbonate solution with tweezers. Rinse with deionized H2O and dry film on paper towel(dabbing gently).
  12. Weigh PVOH film. Record mass= .8059g

General Protocol 2 (Noah):

  1. Repeat Glutaraldehyde film prep
  2. make 2nd glutaradehyde film film, except and replace 10% PVOH with PDMS
  3. Prepare 1 L glutaraldehyde solutions
  4. prepare 500ml 20ppm SO42- as CuSO4
  5. prepare 500ml 20ppm SO42- as Na2SO4
  6. dry clays

Glutaraldehyde Prep and CuSO4*5H20 20ppm

  • 0.5g PVOH, 5ml H2O => dissolve
  • add 1ml Glutaraldehyde in fume hood
  • 0.5132 g PVOH, 5ml h2o + 1ml glutaraldehyde
  • 0.4055g PVOH, 0.1015 PDMS, 5ml h2o

*200 g Na2So4, 1L H2O stirred to dissolution + 20ml H2 So4 *MW CuSO4 * 5 H20= 249.67 g/mole 20 ppm in 500ml H2O MW= 18 g/mol (500g of h20/18g/mol=27.7moles) **27.7moles h2O* ((20moles cuso4*5H2O)/1,000,000 moles H2O) = 5.55 E -4 moles Cuso4*5molesH20 **5.55E-4moles CuSo4*5H2O * 249.68 g/mol= 0.1387g CuSo4*5H2O **5.55E-4moles Na2SO4 * 142.04g/mol=0.078 (20ppm) *we want 500ml of 20ppm = 20mg/l CuSO4 ** 277.4mg/l*x=.5*20mg/l ** x=36.05ml in total 100ml solution * we want 500ml of 20ppm= 20mg/l Na2SO4 ** 156.8 mg/l x = .5(20mg/L)v <s>** x= 63.78ml in total 100ml solution

Data

  • Add data and results here...

Notes

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