User:Klare Lazor/Notebook/Chem-496-001/2012/09/19: Difference between revisions
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==Objective== | ==Objective== | ||
#Crosslink the prepared glutaraldehyde film | #Crosslink the prepared glutaraldehyde film | ||
#Repeat the crosslink of glutaraldehyde film | |||
# Repeat the crosslink of glutaraldehyde film | |||
==Description== | ==Description== | ||
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#After 30 minutes, remove film from 1% HCl solution and place in 0.2M sodium bicarbonate solution for ~15minutes | #After 30 minutes, remove film from 1% HCl solution and place in 0.2M sodium bicarbonate solution for ~15minutes | ||
#Remove film from dilute sodium bicarbonate solution with tweezers. Rinse with deionized H<sub>2</sub>O and dry film on paper towel(dabbing gently). | #Remove film from dilute sodium bicarbonate solution with tweezers. Rinse with deionized H<sub>2</sub>O and dry film on paper towel(dabbing gently). | ||
#Weigh PVOH film. Record mass | #Weigh PVOH film. Record mass= .8059g | ||
General Protocol 2 (Noah): | General Protocol 2 (Noah): | ||
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#dry clays | #dry clays | ||
'''Glutaraldehyde Prep''' | '''Glutaraldehyde Prep and CuSO4*5H20 20ppm''' | ||
* 0.5g PVOH, 5ml H2O => dissolve | |||
* add 1ml Glutaraldehyde in fume hood | |||
*0.5132 g PVOH, 5ml h2o + 1ml glutaraldehyde | |||
*0.4055g PVOH, 0.1015 PDMS, 5ml h2o | |||
<s>*200 g Na2So4, 1L H2O stirred to dissolution + 20ml H2 So4 </s> | |||
<s>*MW CuSO4 * 5 H20= 249.67 g/mole 20 ppm in 500ml H2O MW= 18 g/mol (500g of h20/18g/mol=27.7moles) </s> | |||
**27.7moles h2O *(20moles | <s>**27.7moles h2O* ((20moles cuso4*5H2O)/1,000,000 moles H2O) = 5.55 E -4 moles Cuso4*5molesH20 </s> | ||
<s>**5.55E-4moles CuSo4*5H2O * 249.68 g/mol= 0.1387g CuSo4*5H2O </s> | |||
<s>**5.55E-4moles Na2SO4 * 142.04g/mol=0.078 (20ppm) </s> | |||
<s>*we want 500ml of 20ppm = 20mg/l CuSO4</s> | |||
<s>** 277.4mg/l*x=.5*20mg/l</s> | |||
<s>** x=36.05ml in total 100ml solution</s> | |||
<s>* we want 500ml of 20ppm= 20mg/l Na2SO4</s> | |||
<s>** 156.8 mg/l x = .5(20mg/L)v | |||
<s>** x= 63.78ml in total 100ml solution</s> | |||
==Data== | ==Data== |
Revision as of 09:11, 7 November 2012
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Objective
DescriptionGeneral Protocol 1(klare and Liz):
General Protocol 2 (Noah):
Glutaraldehyde Prep and CuSO4*5H20 20ppm
Data
NotesThis area is for any observations or conclusions that you would like to note.
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