User:Klare Lazor/Notebook/Chem-496-001/2012/09/19: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | ==Objective== | ||
#Crosslink the prepared glutaraldehyde film | #Crosslink the prepared glutaraldehyde film | ||
#Repeat the crosslink of glutaraldehyde film | |||
# Repeat the crosslink of glutaraldehyde film | |||
==Description== | ==Description== | ||
General Protocol 1(klare and Liz): | General Protocol 1(klare and Liz): | ||
#Allow the glutaraldehyde PVOH film to completely air dry | #Allow the glutaraldehyde PVOH film to completely air dry | ||
#Prepare a solution of 100mL H<sub>2</sub>O, 2mL concentrated H<sub>2</sub>SO<sub>4</sub>, and 20g Na<sub>2</sub>SO<sub>4</sub> in a 150mL beaker. Place stir bar in beaker and a Buchner funnel top over the stir bar | #Prepare a solution of 100mL H<sub>2</sub>O, 2mL concentrated H<sub>2</sub>SO<sub>4</sub>, and 20g Na<sub>2</sub>SO<sub>4</sub> in a 150mL beaker. Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar | ||
#Heat the prepared solution to 70°C(on hot plate turn temperature to 160°C) | #Heat the prepared solution to 70°C(on hot plate turn temperature to 160°C) | ||
#Place film in solution for 1 hour.(Make sure the temperature remains at 70°C) | #Place film in solution for 1 hour.(Make sure the temperature remains at 70°C) '''Must be under fumehood because of glutaraldehyde''' | ||
#Prepare 100mL 1% aqueous HCl solution. Heat solution to 70°C | #Prepare 100mL 1% aqueous HCl solution. Heat solution to 70°C. (ie: 1ml of HCL in 100 ml) | ||
#Remove film from the solution and rinse with deionized H<sub>2</sub>O | #Remove film from the solution and rinse with deionized H<sub>2</sub>O | ||
#Place stir bar in 1% HCl solution and a Buchner funnel top over of the stir bar. Add the PVOH film to the solution | #Place stir bar in 1% HCl solution and a Buchner funnel top over of the stir bar. Add the PVOH film to the solution | ||
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#After 30 minutes, remove film from 1% HCl solution and place in 0.2M sodium bicarbonate solution for ~15minutes | #After 30 minutes, remove film from 1% HCl solution and place in 0.2M sodium bicarbonate solution for ~15minutes | ||
#Remove film from dilute sodium bicarbonate solution with tweezers. Rinse with deionized H<sub>2</sub>O and dry film on paper towel(dabbing gently). | #Remove film from dilute sodium bicarbonate solution with tweezers. Rinse with deionized H<sub>2</sub>O and dry film on paper towel(dabbing gently). | ||
#Weigh PVOH film. Record mass | #Weigh PVOH film. Record mass= .8059g | ||
General Protocol 2 (Noah): | General Protocol 2 (Noah): | ||
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#dry clays | #dry clays | ||
'''Glutaraldehyde Prep''' | '''Glutaraldehyde Prep and CuSO4*5H20 20ppm''' | ||
* 0.5g PVOH, 5ml H2O => dissolve | |||
* add 1ml Glutaraldehyde in fume hood | |||
*0.5132 g PVOH | *0.5132 g PVOH, 5ml h2o + 1ml glutaraldehyde | ||
*0.4055g PVOH, 0.1015 PDMS, 5ml h2o | |||
<s>*200 g Na2So4, 1L H2O stirred to dissolution + 20ml H2 So4 </s> | |||
<s>*MW CuSO4 * 5 H20= 249.67 g/mole 20 ppm in 500ml H2O MW= 18 g/mol (500g of h20/18g/mol=27.7moles) </s> | |||
<s>**27.7moles h2O* ((20moles cuso4*5H2O)/1,000,000 moles H2O) = 5.55 E -4 moles Cuso4*5molesH20 </s> | |||
<s>**5.55E-4moles CuSo4*5H2O * 249.68 g/mol= 0.1387g CuSo4*5H2O </s> | |||
<s>**5.55E-4moles Na2SO4 * 142.04g/mol=0.078 (20ppm) </s> | |||
<s>*we want 500ml of 20ppm = 20mg/l CuSO4</s> | |||
<s>** 277.4mg/l*x=.5*20mg/l</s> | |||
<s>** x=36.05ml in total 100ml solution</s> | |||
<s>* we want 500ml of 20ppm= 20mg/l Na2SO4</s> | |||
<s>** 156.8 mg/l x = .5(20mg/L)v | |||
<s>** x= 63.78ml in total 100ml solution</s> | |||
==Data== | ==Data== |
Latest revision as of 22:01, 26 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
Objective
DescriptionGeneral Protocol 1(klare and Liz):
General Protocol 2 (Noah):
Glutaraldehyde Prep and CuSO4*5H20 20ppm
Data
NotesThis area is for any observations or conclusions that you would like to note.
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