User:Klare Lazor/Notebook/Chem-496-001/2012/09/19

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Objective

  1. Crosslink the prepared glutaraldehyde film
  2. Run DSC on 8 films
  3. Run FTIR
  4. Repeat the crosslink of glutaraldehyde film

Description

General Protocol 1(klare and Liz):

  1. Allow the glutaraldehyde PVOH film to completely air dry
  2. Prepare a solution of 100mL H2O, 2mL concentrated H2SO4, and 20g Na2SO4 in a 150mL beaker. Place stir bar in beaker and a Buchner funnel top over the stir bar
  3. Heat the prepared solution to 70°C(on hot plate turn temperature to 160°C)
  4. Place film in solution for 1 hour.(Make sure the temperature remains at 70°C)
  5. Prepare 100mL 1% aqueous HCl solution. Heat solution to 70°C
  6. Remove film from the solution and rinse with deionized H2O
  7. Place stir bar in 1% HCl solution and a Buchner funnel top over of the stir bar. Add the PVOH film to the solution
  8. Allow film to remain in 1% HCl solution for 30 minutes.(Be sure to keep a constant 70°C)
  9. Prepare 0.2M sodium bicarbonate solution
  10. After 30 minutes, remove film from 1% HCl solution and place in 0.2M sodium bicarbonate solution for ~15minutes
  11. Remove film from dilute sodium bicarbonate solution with tweezers. Rinse with deionized H2O and dry film on paper towel(dabbing gently).
  12. Weigh PVOH film. Record mass

General Protocol 2 (Noah):

  1. Repeat Glutaraldehyde film prep
  2. make 2nd glutaradehyde film film, except and replace 10% PVOH with PDMS
  3. Prepare 1 L glutaraldehyde solutions
  4. prepare 500ml 20ppm SO42- as CuSO4
  5. prepare 500ml 20ppm SO42- as Na2SO4
  6. dry clays

Glutaraldehyde Prep

  1. 0.5g PVOH, 5ml H2O => dissolve
  2. add 1ml Glutaraldehyde in fume hood
    • 0.5132 g PVOH

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


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