User:Klare Lazor/Notebook/Chem-496-001/2013/02/04: Difference between revisions
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==Description== | ==Description== | ||
'''Cross-linking PVOH+Isopropyl+glutaraldehdye''' | |||
#Allow the PVOH film containing glutaraldehyde to dry completely. | |||
#Prepare a solution of 100 mL H2O, 2 mL concentrated H2SO4, and 20 g Na2SO4 in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant) | |||
#Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar | |||
#Heat solution to 70°C (temperature on hotplate should be about 160) | |||
#Place film in solution for one hour. | |||
#Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker. | |||
#Heat HCl solution to 70°C | |||
#Remove film from glutaraldehyde fixant and rinse with distilled water. | |||
#Place film into HCl solution for 30 minutes. | |||
#Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL) | |||
#After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes. | |||
#Use tweezers to remove the film from the sodium bicarbonate solution. | |||
#Rinse film with deionized water and place in a beaker with distilled water until it is used/tested | |||
==Data== | ==Data== |
Revision as of 10:51, 4 February 2013
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Objective
DescriptionCross-linking PVOH+Isopropyl+glutaraldehdye
Data
NotesThis area is for any observations or conclusions that you would like to note.
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