User:Klare Lazor/Notebook/Chem-496-001/2013/02/07

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(Objective)
(Objective)
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#Fill 5 50mL beakers with 15mL HPLC water. Place on hot/stir plates in the hood.
#Fill 5 50mL beakers with 15mL HPLC water. Place on hot/stir plates in the hood.
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#Measure out 1g of PVOH (5 times) and place into the 5 beakers (1g each). Allow PVOH to begin dissolving on hot/stir plates.
#Measure out 1g of PVOH (5 times) and place into the 5 beakers (1g each). Allow PVOH to begin dissolving on hot/stir plates.
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#Measure out 2.1457g Phenol, 0.21457g Phenol, 0.021457g Phenol, and 0.00257g Phenol (for the latter I prepared a solution of 10mL and 0.22g Phenol and used 1mL [for 0.022g] and 0.1mL [for 0.0022g]). Add each measurement of phenol to 4 of the beakers with PVOH respectively.
#Measure out 2.1457g Phenol, 0.21457g Phenol, 0.021457g Phenol, and 0.00257g Phenol (for the latter I prepared a solution of 10mL and 0.22g Phenol and used 1mL [for 0.022g] and 0.1mL [for 0.0022g]). Add each measurement of phenol to 4 of the beakers with PVOH respectively.
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#Allow mixtures of PVOH, Phenol, and water to stir on hot/stir plate until fully dissolved.
#Allow mixtures of PVOH, Phenol, and water to stir on hot/stir plate until fully dissolved.
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#Once full dissolution is achieved, add 1mL Glutaraldehyde to each of the 5 beakers. Allow to stir off heat for 2-3 minutes.
#Once full dissolution is achieved, add 1mL Glutaraldehyde to each of the 5 beakers. Allow to stir off heat for 2-3 minutes.
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#Pour solutions into labeled film dishes.
#Pour solutions into labeled film dishes.

Revision as of 17:05, 8 February 2013

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Objective

Making PVOH/Glutaraldehyde/Phenol Films:

  1. Fill 5 50mL beakers with 15mL HPLC water. Place on hot/stir plates in the hood.
  2. Measure out 1g of PVOH (5 times) and place into the 5 beakers (1g each). Allow PVOH to begin dissolving on hot/stir plates.
  3. Measure out 2.1457g Phenol, 0.21457g Phenol, 0.021457g Phenol, and 0.00257g Phenol (for the latter I prepared a solution of 10mL and 0.22g Phenol and used 1mL [for 0.022g] and 0.1mL [for 0.0022g]). Add each measurement of phenol to 4 of the beakers with PVOH respectively.
  4. Allow mixtures of PVOH, Phenol, and water to stir on hot/stir plate until fully dissolved.
  5. Once full dissolution is achieved, add 1mL Glutaraldehyde to each of the 5 beakers. Allow to stir off heat for 2-3 minutes.
  6. Pour solutions into labeled film dishes.

Description

Data

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