User:Klare Lazor/Notebook/Chem-496-001/2013/02/25: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(3 intermediate revisions by the same user not shown)
Line 10: Line 10:


==Objective==
==Objective==
Hydrogels for eventual attachment of protein


==Description==
==Description==


*To eventually attach a protein, we can not crosslink in acidic or heated conditions. Therefore, for this procedure. We will be using hydrogels instead.
'''Procedure'''
 
* 4 hydrogels were prepared.  2 were made using PVOH MW = 22,000, while the other two were made using PVOH MW = 146,000 - 186,000.  For each molecular weight, one hydrogel was made with isopropanol alone, and one was made with isopropanol + glutaraldehyde.  The amounts of isopropanol used is the same as in previous trials.


To eventually attach a protein, we can not crosslink in acidic or heated conditions. Therefore, for this procedure. We will be using hydrogels instead. 4 hydrogels were prepared.  2 were made using PVOH MW = 22,000, while the other two were made using PVOH MW = 146,000 - 186,000.  For each molecular weight, one hydrogel was made with isopropanol alone, and one was made with isopropanol + glutaraldehyde.  The amounts of isopropanol used is the same as in previous trials.
# Measure out 2 samples of 1 g PVOH (MW = 22,000) and 2 samples of 1 g PVOH (MW = 146,000 - 186,000)
# Measure out 2 samples of 1 g PVOH (MW = 22,000) and 2 samples of 1 g PVOH (MW = 146,000 - 186,000)
# Dissolve in 20 mL water (Dissolve on heat)
# Dissolve in 20 mL water (Dissolve on heat)
Line 22: Line 22:
# 1 mL glutaraldehyde was added to the 2 hydrogels that are isopropanol + glut
# 1 mL glutaraldehyde was added to the 2 hydrogels that are isopropanol + glut
# The hydrogels were placed in the freezer for 24 hours out for 4 hours in for 24hours and then out once again. repeated cycles.
# The hydrogels were placed in the freezer for 24 hours out for 4 hours in for 24hours and then out once again. repeated cycles.
'''Calculations'''
2 hydrogels of 22k MW PVOH and 2 hydrogels of 146-186k MW PVOH. All will have 1% isopropanol: PVOH OH groups. 1 of each will have glutaraldehyde (1mL).
From previous calculations, 1g PVOH contains 1.373E22 OH groups. We want 1% of these to react w isopropanol at 1:1. (1.373E22)(0.01)=1.373E20 molecules of isopropanol. (1.373E20)/(6.022E23 molecules per mole)=2.27997E-4 moles isopropanol. (2.27994E-4 moles)(60.1 grams per mole)=0.0137 grams isopropanol. (0.0137g)/(0.786 grams per mL)=0.0174mL Isopropanol.
# 1g 22000mw PVOH dissolved in 20mL distilled water + 0.0174mL Isopropanol once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
# 1g 22000mw PVOH dissolved in 20mL distilled water + 0.0174mL Isopropanol and 1mL glutaraldehyde (in hood) once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
# 1g 146-186K mw PVOH dissolved in 30mL distilled water + 0.0174mL Isopropanol once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
# 1g 146-186K mw PVOH dissolved in 30mL distilled water + 0.0174mL Isopropanol and 1mL glutaraldehyde (in hood) once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.


==Data==
==Data==

Revision as of 21:30, 27 February 2013

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Objective

Hydrogels for eventual attachment of protein

Description

Procedure

To eventually attach a protein, we can not crosslink in acidic or heated conditions. Therefore, for this procedure. We will be using hydrogels instead. 4 hydrogels were prepared. 2 were made using PVOH MW = 22,000, while the other two were made using PVOH MW = 146,000 - 186,000. For each molecular weight, one hydrogel was made with isopropanol alone, and one was made with isopropanol + glutaraldehyde. The amounts of isopropanol used is the same as in previous trials.

  1. Measure out 2 samples of 1 g PVOH (MW = 22,000) and 2 samples of 1 g PVOH (MW = 146,000 - 186,000)
  2. Dissolve in 20 mL water (Dissolve on heat)
  3. Once dissolved, 0.0174 mL isopropanol was added to each sample. The samples were allowed to stir for a few minutes.
  4. 1 mL glutaraldehyde was added to the 2 hydrogels that are isopropanol + glut
  5. The hydrogels were placed in the freezer for 24 hours out for 4 hours in for 24hours and then out once again. repeated cycles.


Calculations

2 hydrogels of 22k MW PVOH and 2 hydrogels of 146-186k MW PVOH. All will have 1% isopropanol: PVOH OH groups. 1 of each will have glutaraldehyde (1mL). From previous calculations, 1g PVOH contains 1.373E22 OH groups. We want 1% of these to react w isopropanol at 1:1. (1.373E22)(0.01)=1.373E20 molecules of isopropanol. (1.373E20)/(6.022E23 molecules per mole)=2.27997E-4 moles isopropanol. (2.27994E-4 moles)(60.1 grams per mole)=0.0137 grams isopropanol. (0.0137g)/(0.786 grams per mL)=0.0174mL Isopropanol.

  1. 1g 22000mw PVOH dissolved in 20mL distilled water + 0.0174mL Isopropanol once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
  2. 1g 22000mw PVOH dissolved in 20mL distilled water + 0.0174mL Isopropanol and 1mL glutaraldehyde (in hood) once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
  3. 1g 146-186K mw PVOH dissolved in 30mL distilled water + 0.0174mL Isopropanol once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.
  4. 1g 146-186K mw PVOH dissolved in 30mL distilled water + 0.0174mL Isopropanol and 1mL glutaraldehyde (in hood) once PVOH fully dissolved. Poured into dish, sealed with parafilm, and placed in freezer.

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Klare_Lazor/Notebook/Chem-496-001/2013/02/25&oldid=679852"