User:Klare Lazor/Notebook/Chem-496-001/2013/03/04: Difference between revisions
From OpenWetWare
Klare Lazor (talk | contribs) |
Klare Lazor (talk | contribs) |
||
(3 intermediate revisions by the same user not shown) | |||
Line 16: | Line 16: | ||
==Description== | ==Description== | ||
'''Autoclave''' | '''Autoclave''' | ||
*8 Flask of 50mL of water and agar. | |||
# Prepare [[AU_Biomaterials_Design_Lab:Materials/LB|LB]] in a 250mL Erlenmeyer flask | |||
# 0.875g of LB | |||
# 35mL of water | |||
# Cover the flask with foil | |||
# Autoclave | |||
# Allow the flask to cool | |||
# Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm | |||
*Separate for starter culture | |||
*Make certain that there is enough water in the reservoir before you begin. | *Make certain that there is enough water in the reservoir before you begin. | ||
# Turn the power on | # Turn the power on | ||
Line 28: | Line 37: | ||
# Turn the black knob to Off. | # Turn the black knob to Off. | ||
# Use gloves to unload your sterilized material. | # Use gloves to unload your sterilized material. | ||
'''Starter Culture''' | |||
# Inoculate with the bacteria you are culturing using one of the two following methods | |||
# Using sterile methods, retrieve a single colony from a petrie dish and drop it into the flask | |||
# Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm | |||
"made plates" | |||
==Data== | ==Data== |
Revision as of 12:05, 8 March 2013
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
DescriptionAutoclave
Starter Culture
"made plates" Data
NotesThis area is for any observations or conclusions that you would like to note.
|