User:Klare Lazor/Notebook/Chem-496-001/2013/03/04: Difference between revisions
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# Allow the flask to cool | # Allow the flask to cool | ||
# Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm | # Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm | ||
*Separate for starter culture | |||
*Make certain that there is enough water in the reservoir before you begin. | *Make certain that there is enough water in the reservoir before you begin. | ||
# Turn the power on | # Turn the power on | ||
Line 36: | Line 37: | ||
# Turn the black knob to Off. | # Turn the black knob to Off. | ||
# Use gloves to unload your sterilized material. | # Use gloves to unload your sterilized material. | ||
'''Starter Culture''' | |||
# Inoculate with the bacteria you are culturing using one of the two following methods | |||
# Using sterile methods, retrieve a single colony from a petrie dish and drop it into the flask | |||
# Place the flask in a shaker/incubator and culture overnight at 37C and 200rpm | |||
==Data== | ==Data== |
Revision as of 12:00, 8 March 2013
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Objective
DescriptionAutoclave
Starter Culture
Data
NotesThis area is for any observations or conclusions that you would like to note.
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