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Objective
Copper catalyzed click reaction with azide flor
Description
Hydrogels
- copper catalyzed click run with azide flor
- dye in dmso, add a lithe and mix with film in water
- add Cu(I) react for over 30 mins @ room temp
- Cu(I) is unstable so add CUSO4 (Cu(II)) and ascorbic acid.
copper concentrations
- biocon:0.375mmol cu(mecn)4PF5, 1.7 mol% cu with respect to total alkyne stirred at room temp for 2 days
- nihms- 1888395: 0.05 mmol CuI (9.52mg).
- PNAS- 2008-salic: From Edu staining section of methods: 0.5-1mM CuSO4, 1-60µM fluorescent azide (in 10-100mM stock in DMSO) + 50-100mM ascorbic acid, (added last, they made a 0.5 M stock solution in water)
- PNAS-2006- Dietracric: 200MM triazle ligand, 50 mM alkyne tag: 100mg/ml Cu(I)Br
- 1m hoding dye in DMS and leave overnight
Calculations for amount of FLOUR
- 17.25µL Butyne-1-ol in the hydrogele
- 17.24µL/70.09g/mol * 0.926g/mol= 2.28E-4 moles of butane-1-ol
- rxn 1:1 however we want 1.5 excess
- azide flour 488 mw: 563.45g/mol
- 564:45g/mol *2.28E-4mol=0.128g*1.5= 0.193 grams
- HOWEVER WE ONLY HAVE 5mg, not enough of the dye, so we will cut off a portion of the hydrogel so decided to take a piece off of it
Mass of whole hydrogel ~12.1219 piece~ 0.8810 g Percent of original film= 7.26%
orginal amount of dye needed~0.193g new amount~ 0.014g
So, tested different concentrations of dye 200, 500, 100, 10, 1µL of dye from stock solution of 5mg in 1ml of dmso
Reaction
- react 1mMCuSO4, 100mM L-ascorbic acid, dye in a total volume of 10ml
Data
- Add data and results here...
Notes
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Biomaterials Design Lab
