User:Klare Lazor/Notebook/Chem-496-001/2013/04/03: Difference between revisions
From OpenWetWare
Klare Lazor (talk | contribs) (Autocreate 2013/04/03 Entry for User:Klare_Lazor/Notebook/Chem-496-001) |
(fix raw html notebook nav) |
||
(5 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 10: | Line 10: | ||
==Objective== | ==Objective== | ||
Hydrogels: Copper catalyzed click rxn with azide flor | |||
==Description== | ==Description== | ||
#dye in dmso, add a little and mix with film in water | |||
#Let dye, ascorbic, cuso4 and hydrogel solution over night to react with dye | |||
#Cu(I) is unstable so add CuSO4 (Cu(II)) and ascorbic acid | |||
# after 24 hours take a uv-vis of the hydrogel soaked in a known volume of dH20 | |||
'''Dye Calculatations''' | |||
* 17.24µL butyne-1-ol in the hydrogel | |||
* (17.24/70.09)*.926= 2.08E-4 moles Butyne-1-ol | |||
*rxn is 1:1 however we want 1.5 * excess of dye | |||
*Dye= azide-flour 488: mw 563.35g/mol | |||
* 563.25g/mol * 2.28E-4moes = 1.28g * 1.5 =0.193 g | |||
**not enough dye so we cut off a portion of the hydrogel to use and dye | |||
'''Mass of cut hydrogel''' | |||
* mass of whole= 12.1419 g | |||
* mass of cut= 0.8810 | |||
* percent of whole = 7.26% | |||
*Original amount of dye needed= 0.014g | |||
** however, we only have 0.005 g | |||
'''Copper sulfat concentration''' | |||
*249.8*0.001moles/l*0.01l= 0.0025 g of copper sulfate | |||
*MADE A STOCK SOLUTION OF .025g/ml so 100µl =0.0025g of copper sulfate | |||
'''L-Ascorbic acid''' | |||
* 176.13g/mol * .1mol/L * 0.0L= 0.1761g | |||
*made stock solution of 1.761g per 10ml so 1 ml = .1761g | |||
'''range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso''' | |||
*ranges: 500, 200, 100, 10 and 1 µL of dye | |||
* Added to hydrogels 9 ml of water + dye + 100µL of copper sulfate + 1 ml of ascorbic acid | |||
==Data== | ==Data== |
Latest revision as of 22:35, 26 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
ObjectiveHydrogels: Copper catalyzed click rxn with azide flor Description
Dye Calculatations
Mass of cut hydrogel
Copper sulfat concentration
L-Ascorbic acid
range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso
Data
NotesThis area is for any observations or conclusions that you would like to note.
|