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Objective
Hydrogels: Copper catalyzed click rxn with azide flor
Description
- dye in dmso, add a little and mix with film in water
- Add Cu(I) react for over 30 mins @ room temp
- Cu(I) is unstable so add CuSO4 (Cu(II)) and ascorbic acid
Dye Calculatations
- 17.24µL butyne-1-ol in the hydrogel
- (17.24/70.09)*.926= 2.08E-4 moles Butyne-1-ol
- rxn is 1:1 however we want 1.5 * excess of dye
- Dye= azide-flour 488: mw 563.35g/mol
- 563.25g/mol * 2.28E-4moes = 1.28g * 1.5 =0.193 g
- not enough dye so we cut off a portion of the hydrogel to use and dye
Mass of cut hydrogel
- mass of whole= 12.1419 g
- mass of cut= 0.8810
- percent of whole = 7.26%
- Original amount of dye needed= 0.014g
- however, we only have 0.005 g
Copper sulfat concentration
- 249.8*0.001moles/l*0.01l= 0.0025 g of copper sulfate
- MADE A STOCK SOLUTION OF .025g/ml so 100µl =0.0025g of copper sulfate
L-Ascorbic acid
- 176.13g/mol * .1mol/L * 0.0L= 0.1761g
- made stock solution of 1.761g per 10ml so 1 ml = .1761g
range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso
- ranges: 500, 200, 100, 10 and 1 µL of dye
- Added to hydrogels 9 ml of water + dye + 100µL of copper sulfate + 1 ml of ascorbic acid
Data
- Add data and results here...
Notes
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Biomaterials Design Lab
