# User:Klare Lazor/Notebook/Chem-496-001/2013/04/03

(Difference between revisions)
 Revision as of 11:00, 3 April 2013 (view source) (→Objective)← Previous diff Current revision (11:17, 3 April 2013) (view source) (→Description) (3 intermediate revisions not shown.) Line 13: Line 13: ==Description== ==Description== + #dye in dmso, add a little and mix with film in water + #Let dye, ascorbic, cuso4 and hydrogel solution over night to react with dye + #Cu(I) is unstable so add CuSO4 (Cu(II)) and ascorbic acid + # after 24 hours take a uv-vis of the hydrogel soaked in a known volume of dH20 + + '''Dye Calculatations''' + * 17.24µL butyne-1-ol in the hydrogel + * (17.24/70.09)*.926= 2.08E-4 moles Butyne-1-ol + *rxn is 1:1 however we want 1.5 * excess of dye + *Dye= azide-flour 488: mw 563.35g/mol + * 563.25g/mol * 2.28E-4moes = 1.28g * 1.5 =0.193 g + **not enough dye so we cut off a portion of the hydrogel to use and dye + + '''Mass of cut hydrogel''' + * mass of whole= 12.1419 g + * mass of cut= 0.8810 + * percent of whole = 7.26% + *Original amount of dye needed= 0.014g + ** however, we only have 0.005 g + + '''Copper sulfat concentration''' + *249.8*0.001moles/l*0.01l= 0.0025 g of copper sulfate + *MADE A STOCK SOLUTION OF .025g/ml so 100µl =0.0025g of copper sulfate + + '''L-Ascorbic acid''' + * 176.13g/mol * .1mol/L * 0.0L= 0.1761g + *made stock solution of 1.761g per 10ml so 1 ml = .1761g + + '''range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso''' + *ranges: 500, 200, 100, 10 and 1 µL of dye + * Added to hydrogels 9 ml of water + dye + 100µL of copper sulfate + 1 ml of ascorbic acid ==Data== ==Data==

## Current revision

Biomaterials Design Lab Main project page
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## Objective

Hydrogels: Copper catalyzed click rxn with azide flor

## Description

1. dye in dmso, add a little and mix with film in water
2. Let dye, ascorbic, cuso4 and hydrogel solution over night to react with dye
3. Cu(I) is unstable so add CuSO4 (Cu(II)) and ascorbic acid
4. after 24 hours take a uv-vis of the hydrogel soaked in a known volume of dH20

Dye Calculatations

• 17.24µL butyne-1-ol in the hydrogel
• rxn is 1:1 however we want 1.5 * excess of dye
• Dye= azide-flour 488: mw 563.35g/mol
• 563.25g/mol * 2.28E-4moes = 1.28g * 1.5 =0.193 g
• not enough dye so we cut off a portion of the hydrogel to use and dye

Mass of cut hydrogel

• mass of whole= 12.1419 g
• mass of cut= 0.8810
• percent of whole = 7.26%
• Original amount of dye needed= 0.014g
• however, we only have 0.005 g

Copper sulfat concentration

• 249.8*0.001moles/l*0.01l= 0.0025 g of copper sulfate
• MADE A STOCK SOLUTION OF .025g/ml so 100µl =0.0025g of copper sulfate

L-Ascorbic acid

• 176.13g/mol * .1mol/L * 0.0L= 0.1761g
• made stock solution of 1.761g per 10ml so 1 ml = .1761g

range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso

• ranges: 500, 200, 100, 10 and 1 µL of dye
• Added to hydrogels 9 ml of water + dye + 100µL of copper sulfate + 1 ml of ascorbic acid

## Data

• Add data and results here...

## Notes

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