# User:Klare Lazor/Notebook/Chem-496-001/2013/04/03

(Difference between revisions)
 Revision as of 11:15, 3 April 2013 (view source) (→Description)← Previous diff Revision as of 11:17, 3 April 2013 (view source) (→Description)Next diff → Line 14: Line 14: ==Description== ==Description== #dye in dmso, add a little and mix with film in water #dye in dmso, add a little and mix with film in water - #Add Cu(I) react for over 30 mins @ room temp + #Let dye, ascorbic, cuso4 and hydrogel solution over night to react with dye #Cu(I) is unstable so add CuSO4 (Cu(II)) and ascorbic acid #Cu(I) is unstable so add CuSO4 (Cu(II)) and ascorbic acid + # after 25 hours take a uv-vis of the hydrogel soaked in a known volume of dH20 '''Dye Calculatations''' '''Dye Calculatations'''

## Revision as of 11:17, 3 April 2013

Biomaterials Design Lab Main project page
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## Objective

Hydrogels: Copper catalyzed click rxn with azide flor

## Description

1. dye in dmso, add a little and mix with film in water
2. Let dye, ascorbic, cuso4 and hydrogel solution over night to react with dye
3. Cu(I) is unstable so add CuSO4 (Cu(II)) and ascorbic acid
4. after 25 hours take a uv-vis of the hydrogel soaked in a known volume of dH20

Dye Calculatations

• 17.24µL butyne-1-ol in the hydrogel
• rxn is 1:1 however we want 1.5 * excess of dye
• Dye= azide-flour 488: mw 563.35g/mol
• 563.25g/mol * 2.28E-4moes = 1.28g * 1.5 =0.193 g
• not enough dye so we cut off a portion of the hydrogel to use and dye

Mass of cut hydrogel

• mass of whole= 12.1419 g
• mass of cut= 0.8810
• percent of whole = 7.26%
• Original amount of dye needed= 0.014g
• however, we only have 0.005 g

Copper sulfat concentration

• 249.8*0.001moles/l*0.01l= 0.0025 g of copper sulfate
• MADE A STOCK SOLUTION OF .025g/ml so 100µl =0.0025g of copper sulfate

L-Ascorbic acid

• 176.13g/mol * .1mol/L * 0.0L= 0.1761g
• made stock solution of 1.761g per 10ml so 1 ml = .1761g

range of dye volumes tested since did not have enough made a stock solution of 5 mg of dye in 1 ml of dmso

• ranges: 500, 200, 100, 10 and 1 µL of dye
• Added to hydrogels 9 ml of water + dye + 100µL of copper sulfate + 1 ml of ascorbic acid

## Data

• Add data and results here...

## Notes

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