User:Klare Lazor/Notebook/Chem-496-001/2013/04/12: Difference between revisions

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*after soaking for 24hours with gel: 1.34E-9 mols
*after soaking for 24hours with gel: 1.34E-9 mols
*leached in water solution after 24 hours: 4.3919E-9 mols
*leached in water solution after 24 hours: 4.3919E-9 mols
**8.9E-7mols- 1.34E-7mols=8.88E-7mols of dye soaked up by gel in 24 hours
**8.9E-9mols- 1.34E-9mols=7.56E-9mols of dye soaked up by gel in 24 hours
** 8.88E-7mols - 4.3919E-9mols= '''8.83E-7 mols of dye attached to the hydrogel actual'''
** 7.56E-9mols - 4.3919E-9mols= '''3.17E-9 mols of dye attached to the hydrogel actual'''
** theoretical mols of attached:
** theoretical mols of attached:  
*Percent yield= actual/theoretical*100=


'''10µL of Dye'''
'''10µL of Dye'''
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**8.9E-8 mols- 8.8E-9mols= 8.03E-8 mols  of dye soaked up by gel in 24 hours
**8.9E-8 mols- 8.8E-9mols= 8.03E-8 mols  of dye soaked up by gel in 24 hours
** 8.03E-8mols - 3.86E-9mols= '''7.64E-8 of dye attached to the hydrogel actual'''
** 8.03E-8mols - 3.86E-9mols= '''7.64E-8 of dye attached to the hydrogel actual'''
** theoretical mols of attached:0.000017 mols dye should bind
** theoretical mols of attached:
*Percent yield=actual/theoretical*100=




'''100µL of Dye'''
'''100µL of Dye'''
number of mols of dye
number of mols of dye
*starting solution: 8.9E-7
*starting solution: 8.9E-7mols
*after soaking for 24hours with gel:  
*after soaking for 24hours with gel: 1.608E-7mols
*leached in water solution after 24 hours:  
*leached in water solution after 24 hours: 3.856E-9 mols
**8.9E-8 mols- 8.8E-9mols=   of dye soaked up by gel in 24 hours
**8.9E-7 mols- 1.608E-7mols= 7.3E-7 mols of dye soaked up by gel in 24 hours
** 8.03E-8mols - 3.86E-9mols= ''' of dye attached to the hydrogel actual'''
** 7.3E-7mols - 3.86E-7mols= '''7.26E-7 of dye attached to the hydrogel actual'''
** theoretical mols of attached:
** theoretical mols of attached:
*Percent yield=actual/theoretical:
'''500µl of dye'''


==Data==
==Data==

Revision as of 17:42, 15 April 2013

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Objective

  • Read plates
  • took florescence of the differ concentrations used with hydrogel pieces and determined the concentrations/mols of dye attached

Description

1µL of Dye number of mols of dye

  • starting solution: 8.9E-9 mols
  • after soaking for 24hours with gel: 1.34E-9 mols
  • leached in water solution after 24 hours: 4.3919E-9 mols
    • 8.9E-9mols- 1.34E-9mols=7.56E-9mols of dye soaked up by gel in 24 hours
    • 7.56E-9mols - 4.3919E-9mols= 3.17E-9 mols of dye attached to the hydrogel actual
    • theoretical mols of attached:
  • Percent yield= actual/theoretical*100=

10µL of Dye number of mols of dye

  • starting solution: 8.9E-8
  • after soaking for 24hours with gel: 8.8E-9 mols
  • leached in water solution after 24 hours: 3.86E-9
    • 8.9E-8 mols- 8.8E-9mols= 8.03E-8 mols of dye soaked up by gel in 24 hours
    • 8.03E-8mols - 3.86E-9mols= 7.64E-8 of dye attached to the hydrogel actual
    • theoretical mols of attached:
  • Percent yield=actual/theoretical*100=


100µL of Dye number of mols of dye

  • starting solution: 8.9E-7mols
  • after soaking for 24hours with gel: 1.608E-7mols
  • leached in water solution after 24 hours: 3.856E-9 mols
    • 8.9E-7 mols- 1.608E-7mols= 7.3E-7 mols of dye soaked up by gel in 24 hours
    • 7.3E-7mols - 3.86E-7mols= 7.26E-7 of dye attached to the hydrogel actual
    • theoretical mols of attached:
  • Percent yield=actual/theoretical:

500µl of dye

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.


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