User:Korey Griffin: Difference between revisions
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*[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide] | *[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide] | ||
*[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization] | *[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization] | ||
*[http:// | *[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extract Preparation] | ||
*[http:// | *[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin Immunohistochemistry Paraffin] | ||
*[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining Immunofluorescence Cell Staining] | *[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining Immunofluorescence Cell Staining] | ||
*[http:// | *[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining] | ||
*[http:// | *[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing] | ||
*[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Sodium Orthovanadate Activation] | *[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Sodium Orthovanadate Activation] | ||
[http:// | *[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios Antibody Protein Coupling (F:P) Ratios] | ||
*[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Antibody Elution Buffers] | *[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Antibody Elution Buffers] | ||
*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics] | *[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics] | ||
Revision as of 10:27, 22 September 2008
Contact Info
- Korey Griffin
- Santa Cruz Biotechnology, Inc.
- 2145 Delaware Avenue Santa Cruz, CA 95060
- Santa Cruz, California, USA
- Email me through OpenWetWare
I am currently a technical support representative for Santa Cruz Biotechnology, Inc. My primary focus is building relationships with industry and academic institutions for anyone from undergrad/graduate students to post-doctoral researchers alike, who are running new experiments for the first time or trying out new antibodies.
Experienced technicians in all aspects of research become so skilled at bench techniques that the nuances of these procedures become second nature, and often times the nuances are what influence the quality and consistency of obtaining good data over time.
There are emerging new ways for bench scientists to optimize their protocols so that results are more meaningful toward producing publication quality data. Researcher time and labor at the bench is very important to consider when running experiments over time. I am joining OWW in order to contribute research antibody (immunoglobulin) and siRNA/shRNA related protocols in order to facilitate the research experience. When I am not at pondering the nuances of protocol optimization (check useful links below, ie Western Blot Optimization), I enjoy surfing, mt. biking, camping, and pen&ink artwork.
Education
- 2000, MS Biochemistry, University of Missouri-Columbia
Advisor: Gary Weisman Ph.D.
- 1997, BS, University of Missouri-Columbia
Research interests
- Helping scientists succeed with research antibodies
- Antibody protocol optimization and imaging
- siRNA & shRNA design & experimental methodology
- Antibody design & experimental methodology
Useful links
- Immunoprecipitation Guide
- Western Blotting Guide
- Western Blot Optimization
- Nuclear Extract Preparation
- Immunohistochemistry Paraffin
- Immunofluorescence Cell Staining
- Dual Immunofluorescence Cell Staining
- Membrane Stripping and Reprobing
- Sodium Orthovanadate Activation
- Antibody Protein Coupling (F:P) Ratios
- Antibody Elution Buffers
- Antibody Basics
Publications
- Liu J, Liao Z, Camden J, Griffin KD, Garrad RC, Santiago-Pérez LI, González FA, Seye CI, Weisman GA, and Erb L. Src homology 3 binding sites in the P2Y2 nucleotide receptor interact with Src and regulate activities of Src, proline-rich tyrosine kinase 2, and growth factor receptors. J Biol Chem. 2004 Feb 27;279(9):8212-8. DOI:10.1074/jbc.M312230200 |
- Erb L, Liu J, Ockerhausen J, Kong Q, Garrad RC, Griffin K, Neal C, Krugh B, Santiago-Pérez LI, González FA, Gresham HD, Turner JT, and Weisman GA. An RGD sequence in the P2Y(2) receptor interacts with alpha(V)beta(3) integrins and is required for G(o)-mediated signal transduction. J Cell Biol. 2001 Apr 30;153(3):491-501. DOI:10.1083/jcb.153.3.491 |
