User:Korey Griffin: Difference between revisions
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*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]] | *[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]] | ||
Bench research leads to advancements in human health and awareness. Confidence in reagent quality & protocol is essential to success in molecular & cell biology. | Bench research leads to advancements in human health and awareness. Confidence in reagent quality & protocol is essential to success in molecular & cell biology. There is an ongoing need to facilitate scientists in creating successful R&D efforts in every phase; through 2-way communication on reagent quality & procedure optimization. | ||
Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward procedures (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of resources for Immunoglobulin and RNAi studies, that improve awareness & success. | Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward procedures (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of resources for Immunoglobulin and RNAi studies, that improve awareness & success. | ||
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*[http://www.proteinatlas.org/ Human Protein Atlas] | *[http://www.proteinatlas.org/ Human Protein Atlas] | ||
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm] | *[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm] | ||
[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]] | [[Image:northcounty.jpg|thumb|right|North County Santa Cruz]] | ||
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]] | [[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]] | ||
Revision as of 13:40, 9 November 2012
Contact
- Korey Griffin MS
- Santa Cruz Biotechnology, Inc.
- Email me through OpenWetWare
Bench research leads to advancements in human health and awareness. Confidence in reagent quality & protocol is essential to success in molecular & cell biology. There is an ongoing need to facilitate scientists in creating successful R&D efforts in every phase; through 2-way communication on reagent quality & procedure optimization.
Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward procedures (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of resources for Immunoglobulin and RNAi studies, that improve awareness & success.
Methods & Techniques
- Antibody Basics
- Antibody Peptide Neutralization
- Antibody Protein Coupling (F:P) Ratios
- Antibody Elution Buffers
- Antibody Related Solutions & Recipes
- Antigen Retrieval Technique
- Chromatin Immunoprecipitation
- Dual Immunofluorescence Cell Staining
- Flow Cytometry prep & labeling
- Immunoprecipitation Guide
- Immunohistochemistry Paraffin
- Immunofluorescence Cell Staining
- In Vitro Kinase Assay
- Isolate Leukocytes from whole blood
- Lentivirus Technology
- Membrane Stripping and Reprobing
- Nested RT-PCR
- Nuclear Extract Preparation
- Puromycin Selection for shRNA
- shRNA Transfection
- siRNA Transfection
- Sodium Orthovanadate Activation
- Western Blotting Guide
- Western Blot Optimization
- Zinc Fixation
Links
- ATCC Cell Lines
- Organelle and Lineage Markers
- DOT guidelines
- Fluorochrome Table
- Human Protein Atlas
- Oligo Tm
