User:Korey Griffin: Difference between revisions

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==Contact Info==
==Contact==
[[Image:OWWEmblem.png|thumb|right|Korey Griffin (an artistic interpretation)]]


*Korey Griffin
[[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]
 
[[Image:halfdome.jpg|thumb|right|Half Dome]]
 
*Korey Griffin MS
*Santa Cruz Biotechnology, Inc.
*Santa Cruz Biotechnology, Inc.
*2145 Delaware Avenue Santa Cruz, CA 95060
*Santa Cruz, California, USA
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]


I am a sales and technical support representative for [http://www.scbt.com/ Santa Cruz Biotechnology, Inc]. My primary focus is building relationships with industry and academic institutions extending beyond the products themselves. Researcher time and labor at the bench is very important to consider when running experiments over time. I am joining OWW in order to contribute research antibody (immunoglobulin) and siRNA/shRNA related protocols in order to facilitate the research experience.
Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization.
 
Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of resources for immunoglobulin and RNAi studies, that improve awareness & success.
 
==Methods & Techniques==


Value your time, know the reagents & have confidence in your technique.
[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]]


Please feel free to call or email me at any time regarding Antibody/RNAi related questions or Issues.
[[Image:Kingscanyon.jpg|thumb|right|Kings Canyon]]


==Education==
[[Image:Nisenemarks.jpg|thumb|right|Sand Point]]
<!--Include info about your educational background-->


* 2000, MS Biochemistry, University of Missouri-Columbia
*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics]
Advisor: [http://biochem.missouri.edu/gweisman.php Gary Weisman Ph.D.]
*[http://openwetware.org/wiki/Griffin:Antibody_Peptide_Neutralization Antibody Peptide Neutralization]
*[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios Antibody Protein Coupling (F:P) Ratios]
*[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Antibody Elution Buffers]
*[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Antibody Related Solutions & Recipes]
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
*[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium Preparation (Growth Factor/Cytokine)]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
*[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide  Immunoprecipitation Guide]
*[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin Immunohistochemistry Paraffin]
*[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
*[http://openwetware.org/wiki/Griffin:Nested_RT-PCR Nested RT-PCR]
*[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extract Preparation]
*[http://openwetware.org/wiki/Griffin:Puromycin_Selection Puromycin Selection for shRNA]
*[http://openwetware.org/wiki/Griffin:shRNA_Transfection shRNA Transfection]
*[http://openwetware.org/wiki/Griffin:siRNA_transfection siRNA Transfection]
*[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Sodium Orthovanadate Activation]
*[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide]
*[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization]
*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]


* 1997, BS, University of Missouri-Columbia
==Links==


==Research interests==
*[http://www.atcc.org ATCC Cell Lines]
<!-- Feel free to add brief descriptions to your research interests as well -->
*[http://www.antibodybeyond.com/ Organelle and Lineage Markers]
# Communication between antibody/reagent vendors and bench scientists
*[http://safety.science.tamu.edu/dot.html DOT guidelines]
# Antibody protocol optimization and imaging
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
# siRNA & shRNA design & experimental methodology
*[http://www.proteinatlas.org/ Human Protein Atlas]
# Antibody design & experimental methodology
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm]


==Publications==
[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications. You can add or remove lines as needed -->
<biblio>
#Paper1 pmid=14670955
#Paper2 pmid=11331301
// This paper made possible in part be a simple and effective way to determine pixel based colocalization counts for dual immunofluorescence labeling
</biblio>


==Useful links==
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[Help|OpenWetWare help pages]]
*[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide  Immunoprecipitation Guide]
*[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide]

Revision as of 11:33, 20 February 2013

Contact

Santa Cruz West cliff
Half Dome

Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization.

Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of resources for immunoglobulin and RNAi studies, that improve awareness & success.

Methods & Techniques

Santa Cruz Mountains
Kings Canyon
Sand Point

Links

North County Santa Cruz
Liberty Cap & Nevada Falls
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