User:Korey Griffin: Difference between revisions

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==Contact Info==
==Contact==
[[Image:image1.jpg|thumb|right|Pondering the nuances of protocol optimization]]


*Korey Griffin
[[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]
 
[[Image:halfdome.jpg|thumb|right|Half Dome]]
 
*Korey Griffin MS
*Santa Cruz Biotechnology, Inc.
*Santa Cruz Biotechnology, Inc.
*2145 Delaware Avenue Santa Cruz, CA 95060
*Santa Cruz, California, USA
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]


I am currently a technical support representative for [http://www.scbt.com/ Santa Cruz Biotechnology, Inc]. My primary focus is building relationships with industry and academic institutions for anyone from undergrad/graduate students to post-doctoral researchers alike, who are running new experiments for the first time or trying out new antibodies.  
Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization.


Experienced technicians in all aspects of research become so skilled at bench techniques that the nuances of these procedures become second nature, and often times the nuances are what influence the quality and consistency of obtaining good data over time.
Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of resources for immunoglobulin and RNAi studies, that improve awareness & success.


There are emerging new ways for bench scientists to optimize their protocols so that results are more meaningful toward producing publication quality data. Researcher time and labor at the bench is very important to consider when running experiments over time. I am joining OWW in order to contribute research antibody (immunoglobulin) and siRNA/shRNA related protocols in order to facilitate the research experience. When I am not at pondering the nuances of protocol optimization (check useful links below, ie [http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization]), I enjoy surfing, mt. biking, camping, and pen&ink artwork.
==Methods & Techniques==


==Education==
[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]]
<!--Include info about your educational background-->


* 2000, MS Biochemistry, [http://www.missouri.edu/ University of Missouri-Columbia]
[[Image:Kingscanyon.jpg|thumb|right|Kings Canyon]]
Advisor: [http://biochem.missouri.edu/gweisman.php Gary Weisman Ph.D.]


* 1997, BS, University of Missouri-Columbia
[[Image:Nisenemarks.jpg|thumb|right|Sand Point]]


==Research interests==
*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics]
<!-- Feel free to add brief descriptions to your research interests as well -->
*[http://openwetware.org/wiki/Griffin:Antibody_Peptide_Neutralization Antibody Peptide Neutralization]
# Helping scientists succeed with research antibodies
*[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios Antibody Protein Coupling (F:P) Ratios]
# Antibody protocol optimization and imaging
*[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Antibody Elution Buffers]
# siRNA & shRNA design & experimental methodology
*[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Antibody Related Solutions & Recipes]
# Antibody design & experimental methodology
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
 
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
==Useful links==
*[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium Preparation (Growth Factor/Cytokine)]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
*[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide  Immunoprecipitation Guide]
*[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide  Immunoprecipitation Guide]
*[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide]
*[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization]
*[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extract Preparation]
*[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin Immunohistochemistry Paraffin]
*[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin Immunohistochemistry Paraffin]
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
*[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
*[http://openwetware.org/wiki/Griffin:Nested_RT-PCR Nested RT-PCR]
*[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extract Preparation]
*[http://openwetware.org/wiki/Griffin:Puromycin_Selection Puromycin Selection for shRNA]
*[http://openwetware.org/wiki/Griffin:shRNA_Transfection shRNA Transfection]
*[http://openwetware.org/wiki/Griffin:siRNA_transfection siRNA Transfection]
*[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Sodium Orthovanadate Activation]
*[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Sodium Orthovanadate Activation]
*[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios Antibody Protein Coupling (F:P) Ratios]
*[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide]
*[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Antibody Elution Buffers]
*[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization]
*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics]
*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]
 
==Links==
 
*[http://www.atcc.org ATCC Cell Lines]
*[http://www.antibodybeyond.com/ Organelle and Lineage Markers]
*[http://safety.science.tamu.edu/dot.html DOT guidelines]
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
*[http://www.proteinatlas.org/ Human Protein Atlas]
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm]


==Publications==
[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications. You can add or remove lines as needed -->
<biblio>
#Paper1 pmid=14670955
#Paper2 pmid=11331301
</biblio>


*[[OpenWetWare:Welcome|Introductory tutorial]]
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
*[[Help|OpenWetWare help pages]]

Revision as of 11:33, 20 February 2013

Contact

Santa Cruz West cliff
Half Dome

Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization.

Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of resources for immunoglobulin and RNAi studies, that improve awareness & success.

Methods & Techniques

Santa Cruz Mountains
Kings Canyon
Sand Point

Links

North County Santa Cruz
Liberty Cap & Nevada Falls
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