User:Korey Griffin: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
(108 intermediate revisions by the same user not shown)
Line 1: Line 1:
==Contact Info==
==Contact==
[[Image:image1.jpg|thumb|right|]]


*Korey Griffin
[[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]
 
[[Image:halfdome.jpg|thumb|right|Half Dome]]
 
*Korey Griffin MS
*Santa Cruz Biotechnology, Inc.
*Santa Cruz Biotechnology, Inc.
*2145 Delaware Avenue Santa Cruz, CA 95060
*Santa Cruz, California, USA
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]


I am currently a technical support representative for [http://www.scbt.com/ Santa Cruz Biotechnology, Inc]. My primary focus is building relationships with researchers who are coordinating Immunoglobulin-based and/or RNAi experiments.  
Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization.
 
Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of resources for immunoglobulin and RNAi studies, that improve awareness & success.


Experienced scientists in molecular/cell biology research eventually become so skillful at bench techniques and protocols, that the nuances of these procedures become second nature, and often times these nuances are what determine the overall quality and consistency of experimental results.
==Methods & Techniques==


There are emerging ways for bench scientists to optimize their protocols so that results are more meaningful toward the ultimate goal of publishing quality data. Time and labor at the bench is very important to consider when running experiments. I am contributing research antibody (immunoglobulin) and siRNA/shRNA related protocols in order to facilitate and provide flow to the research experience.
[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]]


==Methods & Techniques for Immunochemists==
[[Image:Kingscanyon.jpg|thumb|right|Kings Canyon]]


[[Image:Sunset.jpg|thumb|right|Santa Cruz Sunset]]
[[Image:Nisenemarks.jpg|thumb|right|Sand Point]]


*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics]
*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics]
Line 24: Line 27:
*[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Antibody Related Solutions & Recipes]
*[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Antibody Related Solutions & Recipes]
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation ChIP]
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
*[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium Preparation (Growth Factor/Cytokine)]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
Line 32: Line 36:
*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
*[http://openwetware.org/wiki/Isolate_leydig_cells_from_testes Isolate Leydig cells]
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
Line 45: Line 48:
*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]
*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]


==Useful Links==
==Links==


*[http://www.antibodybeyond.com/ Cell Marker]
*[http://www.atcc.org ATCC Cell Lines]
*[http://www.exactantigen.com/ ExactAntigen]
*[http://www.antibodybeyond.com/ Organelle and Lineage Markers]
*[http://www.dsi.univ-paris5.fr/genatlas/ Gene Atlas]
*[http://safety.science.tamu.edu/dot.html DOT guidelines]
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
*[http://www.proteinatlas.org/ Human Protein Atlas]
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm]


==Life==
[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]
 
*2000-2008: Technical Support Scientist, [http://www.scbt.com/ Santa Cruz Biotechnology, Inc.], Santa Cruz CA.
*2007-2008: [http://saveourshores.org/ Save our Shores] Sanctuary Steward, Santa Cruz CA. Save Our Shores is a non-profit marine conservation organization in Santa Cruz, California whose mission is marine conservation through policy research, education, and citizen involvement.
*2006-2007: At-home tutoring for Math, Biology & Chemistry, Santa Cruz CA.
*2005-2006: [http://www.rideawave.org/ Ride-A-Wave volunteer], Santa Cruz CA. RAW is a Santa Cruz based nonprofit org whose mission is to give children with special needs the chance to feel the thrill of riding a wave and experience a safe, fun-filled day at the beach, whether they are physically, developmentally or economically challenged.
*1997: Summer Youth Camp Counselor for [http://www.navigators.org/us/ministries/eaglelake/ Eagle Lake Camp], Pike Forest CO.
*1995,1996: Canoe Guide/Interpreter [http://www.ntier.org/ Charles L. Sommers Canoe Base], Ely MN: Groups are outfitted at the Base to travel by canoe through canoe country, the Boundary Waters Canoe Area Wilderness (BWCAW), Ontario's Quetico Provincial Park, and points beyond. The BWCAW and Quetico together form a roadless area of about a million acres with deep glacial lakes and densely forested cover.
*1994-1997: College Inorganic Chemistry tutor, Columbia MO.
*1993-1997: Sigma Chi Xi Xi chapter, social fraternity, Columbia MO.
*1990: Eagle Scout, Boy Scouts of America, St. Louis MO.
 
==Education==
<!--Include info about your educational background-->
 
* 2000, MS Biochemistry, [http://www.missouri.edu/ University of Missouri-Columbia], Advisor: [http://biochem.missouri.edu/gweisman.php Gary Weisman Ph.D.]
* 1997, BS, University of Missouri-Columbia


==Publications==
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications. You can add or remove lines as needed -->
<biblio>
#Paper1 pmid=14670955
#Paper2 pmid=11331301
</biblio>

Revision as of 11:33, 20 February 2013

Contact

Santa Cruz West cliff
Half Dome

Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization.

Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of resources for immunoglobulin and RNAi studies, that improve awareness & success.

Methods & Techniques

Santa Cruz Mountains
Kings Canyon
Sand Point

Links

North County Santa Cruz
Liberty Cap & Nevada Falls