User:Korey Griffin: Difference between revisions

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==Contact Info==
==Contact==


[[Image:Santa Cruz.jpg|thumb|right|West Side]]
[[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]


[[Image:halfdome.jpg|thumb|right|Half Dome]]
[[Image:halfdome.jpg|thumb|right|Half Dome]]


*Korey Griffin
*Korey Griffin MS
*Santa Cruz Biotechnology, Inc.
*Santa Cruz Biotechnology, Inc.
*2145 Delaware Avenue Santa Cruz, CA 95060
*Santa Cruz, California, USA
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]


I am currently a technical support representative for [http://www.scbt.com/ Santa Cruz Biotechnology, Inc]. My primary focus is in communication with researchers who are coordinating Immunoglobulin-based and/or RNAi experiments with SCBT reagents.  
Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization.


Experienced scientists in molecular/cell biology research eventually through the test of reproducing data become so skillful at bench techniques and protocols, that the nuances of these procedures become second nature. Often times these nuances are what determine the overall quality and consistency of experimental results. Like preparing a gourmet recipe, premium ingredients are essential, though it is the experience of a master chef who determines the quality of the dish.
Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of resources for immunoglobulin and RNAi studies, that improve awareness & success.


There are emerging ways for bench scientists to optimize their protocols, toward the ultimate goal of publishing quality data and expanding on our classical understanding of biological systems. The intrinsic value of time and labor spent at the bench is very important to consider when running molecular/cell experiments since the margin for obtaining a conclusive result is inverse to the number of variables associated with the protocol. And even the most simple of procedures contains an extraordinary variable set. I am contributing research antibody (immunoglobulin) and siRNA/shRNA related information in order to increase awareness for scientists and to optimize the research experience.
==Methods & Techniques==
 
==Methods & Techniques for Immunochemists==


[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]]
[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]]
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*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
*[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium Preparation (Growth Factor/Cytokine)]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
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*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
*[http://openwetware.org/wiki/Isolate_leydig_cells_from_testes Isolate Leydig cells]
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
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*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]
*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]


==Useful Links==
==Links==


[[Image:Sunset.jpg|thumb|right|Santa Cruz Sunset]]
*[http://www.atcc.org ATCC Cell Lines]
 
*[http://www.antibodybeyond.com/ Organelle and Lineage Markers]
*[http://www.antibodybeyond.com/ Cell Marker]
*[http://safety.science.tamu.edu/dot.html DOT guidelines]
*[http://www.exactantigen.com/ ExactAntigen]
*[http://www.dsi.univ-paris5.fr/genatlas/ Gene Atlas]
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
*[http://safety.science.tamu.edu/dot.html DOT guidelines]
*[http://www.proteinatlas.org/ Human Protein Atlas]
 
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm]
==Experience==


[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]
[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]


*2007-2008: [http://saveourshores.org/ Save our Shores] Sanctuary Steward, Santa Cruz CA. Save Our Shores is a non-profit marine conservation organization in Santa Cruz, California whose mission is marine conservation through policy research, education, and citizen involvement.
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
*2006-2007: At-home tutoring for Math, Biology & Chemistry, Santa Cruz CA.
*2005-2006: [http://www.rideawave.org/ Ride-A-Wave volunteer], Santa Cruz CA. RAW is a Santa Cruz based nonprofit org whose mission is to give children with special needs the chance to feel the thrill of riding a wave and experience a safe, fun-filled day at the beach, whether they are physically, developmentally or economically challenged.
*1997: Summer Youth Camp Counselor for [http://www.navigators.org/us/ministries/eaglelake/ Eagle Lake Camp], Pike Forest CO.
*1995,1996: Canoe Guide/Interpreter [http://www.ntier.org/ Charles L. Sommers Canoe Base], Ely MN: Groups are outfitted at the Base to travel by canoe through canoe country, the Boundary Waters Canoe Area Wilderness (BWCAW), Ontario's Quetico Provincial Park, and points beyond. The BWCAW and Quetico together form a roadless area of about a million acres with deep glacial lakes and densely forested cover.
*1993-1997: Sigma Chi, Xi Xi chapter.
*1990: Eagle Scout, Boy Scouts of America.
 
==Education & Publication==
 
[[Image:Vernal Falls.jpg|thumb|right|Nevada Falls]]
 
<!--Include info about your educational background-->
 
* 2000, MS Biochemistry, [http://www.missouri.edu/ University of Missouri-Columbia], Advisor: [http://biochem.missouri.edu/gweisman.php Gary Weisman Ph.D.]
* 1997, BS, University of Missouri-Columbia
 
<biblio>
#Paper1 pmid=14670955
#Paper2 pmid=11331301
</biblio>

Revision as of 11:33, 20 February 2013

Contact

Santa Cruz West cliff
Half Dome

Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization.

Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of resources for immunoglobulin and RNAi studies, that improve awareness & success.

Methods & Techniques

Santa Cruz Mountains
Kings Canyon
Sand Point

Links

North County Santa Cruz
Liberty Cap & Nevada Falls
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