User:Korey Griffin: Difference between revisions

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==Contact Info==
==Contact==


[[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]
[[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]
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[[Image:halfdome.jpg|thumb|right|Half Dome]]
[[Image:halfdome.jpg|thumb|right|Half Dome]]


*Korey Griffin M.S.
*Korey Griffin MS
*Santa Cruz Biotechnology, Inc.
*Santa Cruz Biotechnology, Inc.
*2145 Delaware Avenue Santa Cruz, CA 95060
*800-457-3801 x129
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]


I am currently a technical support representative for [http://www.scbt.com/ Santa Cruz Biotechnology, Inc] where my primary role is communication with researchers in California Institutions who are coordinating chemical, immunoglobulin, & RNAi experiments with SCBT reagents.
Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization.


Researcher time/labor at the bench is important in literally every way. There has to be a high degree of confidence in both the reagent quality, and the procedure. The means to obtain and test quality reagents is beneficial to both the vendor and customer. Let me know what SCBT reagents you have interest in working with and I will do my 100% best to ensure you obtain reagents that produce results.
Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Presenting below, a valuable collection of resources, that will improve awareness & success.


There are several variables for each molecular/cell protocol; apparently straight-forward procedures (ie immunoblotting) contain an extraordinary amount of important steps. Below are resources for research antibody and RNAi studies, that can improve your next experiment, and expand your knowledge. I hope that you may find something useful to bring you success in your next experiment.
==Methods & Techniques==
 
==Methods & Techniques for Immunochemists==


[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]]
[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]]
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*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
*[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium Preparation (Growth Factor/Cytokine)]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
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*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]
*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]


==Useful Links==
==Links==


*[http://www.atcc.org Cell Lines]
*[http://www.atcc.org ATCC Cell Lines]
*[http://www.antibodybeyond.com/ Cell Marker]
*[http://www.antibodybeyond.com/ Organelle and Lineage Markers]
*[http://safety.science.tamu.edu/dot.html DOT guidelines]
*[http://safety.science.tamu.edu/dot.html DOT guidelines]
*[http://www.exactantigen.com/ ExactAntigen]
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
*[http://www.dsi.univ-paris5.fr/genatlas/ Gene Atlas]
*[http://www.proteinatlas.org/ Human Protein Atlas]
*[http://www.proteinatlas.org/ Human Protein Atlas]
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm]
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm]
==Experience==


[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]
[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]


*2007-2009: [http://saveourshores.org/ Save our Shores] Sanctuary Steward, Santa Cruz CA. Save Our Shores is a non-profit marine conservation organization in Santa Cruz, California whose mission is marine conservation through policy research, education, and citizen involvement.
*2005-2006: [http://www.rideawave.org/ Ride-A-Wave volunteer], Santa Cruz CA. RAW is a Santa Cruz based nonprofit org whose mission is to give children with special needs the chance to feel the thrill of riding a wave and experience a safe, fun-filled day at the beach, whether they are physically, developmentally or economically challenged.
*1997: Summer Youth Camp Counselor for [http://www.navigators.org/us/ministries/eaglelake/ Eagle Lake Camp], Pike Forest CO.
*1995,1996: Canoe Guide/Interpreter [http://www.ntier.org/ Charles L. Sommers Canoe Base], Ely MN: Groups are outfitted at the Base to travel by canoe through canoe country, the Boundary Waters Canoe Area Wilderness (BWCAW), Ontario's Quetico Provincial Park, and points beyond. The BWCAW and Quetico together form a roadless area of about a million acres with deep glacial lakes and densely forested cover.
==Education & Publication==
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
* 2000, MS Biochemistry, [http://www.missouri.edu/ University of Missouri-Columbia], Advisor: [http://biochem.missouri.edu/gweisman.php Gary Weisman Ph.D.]
* 1997, BS, University of Missouri-Columbia
==Publications==
J Biol Chem. 2004 Feb 27;279(9):8212-8. Epub 2003 Dec 11. Src homology 3 binding sites in the P2Y2 nucleotide receptor interact with Src and regulate activities of Src, proline-rich tyrosine kinase 2, and growth factor receptors. Liu J, Liao Z, Camden J, Griffin KD, Garrad RC, Santiago-Pérez LI, González FA, Seye CI, Weisman GA, Erb L.  PMID: 14670955
J Cell Biol. 2001 Apr 30;153(3):491-501. An RGD sequence in the P2Y(2) receptor interacts with alpha(V)beta(3) integrins and is required for G(o)-mediated signal transduction. Erb L, Liu J, Ockerhausen J, Kong Q, Garrad RC, Griffin K, Neal C, Krugh B, Santiago-Pérez LI, González FA, Gresham HD, Turner JT, Weisman GA.  PMID: 11331301

Revision as of 17:06, 29 July 2014

Contact

Santa Cruz West cliff
Half Dome

Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization.

Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Presenting below, a valuable collection of resources, that will improve awareness & success.

Methods & Techniques

Santa Cruz Mountains
Kings Canyon
Sand Point

Links

North County Santa Cruz
Liberty Cap & Nevada Falls
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