User:Korey Griffin: Difference between revisions
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==Contact | ==Contact== | ||
[[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]] | [[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]] | ||
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[[Image:halfdome.jpg|thumb|right|Half Dome]] | [[Image:halfdome.jpg|thumb|right|Half Dome]] | ||
*Korey Griffin | *Korey Griffin MS | ||
*Santa Cruz Biotechnology, Inc. | *Santa Cruz Biotechnology, Inc. | ||
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]] | *[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]] | ||
Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization. | |||
Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Presenting below, a valuable collection of resources, that will improve awareness & success. | |||
==Methods & Techniques== | |||
==Methods & Techniques | |||
[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]] | [[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]] | ||
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*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique] | *[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique] | ||
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation] | *[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation] | ||
*[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium Preparation (Growth Factor/Cytokine)] | |||
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining] | *[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining] | ||
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling] | *[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling] | ||
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*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation] | *[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation] | ||
== | ==Links== | ||
*[http://www.atcc.org Cell Lines] | *[http://www.atcc.org ATCC Cell Lines] | ||
*[http://www.antibodybeyond.com/ | *[http://www.antibodybeyond.com/ Organelle and Lineage Markers] | ||
*[http://safety.science.tamu.edu/dot.html DOT guidelines] | *[http://safety.science.tamu.edu/dot.html DOT guidelines] | ||
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table] | *[http://flowcyt.salk.edu/fluo.html Fluorochrome Table] | ||
*[http://www.proteinatlas.org/ Human Protein Atlas] | *[http://www.proteinatlas.org/ Human Protein Atlas] | ||
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm] | *[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm] | ||
[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]] | [[Image:northcounty.jpg|thumb|right|North County Santa Cruz]] | ||
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]] | [[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]] | ||
Revision as of 17:06, 29 July 2014
Contact
- Korey Griffin MS
- Santa Cruz Biotechnology, Inc.
- Email me through OpenWetWare
Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization.
Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Presenting below, a valuable collection of resources, that will improve awareness & success.
Methods & Techniques
- Antibody Basics
- Antibody Peptide Neutralization
- Antibody Protein Coupling (F:P) Ratios
- Antibody Elution Buffers
- Antibody Related Solutions & Recipes
- Antigen Retrieval Technique
- Chromatin Immunoprecipitation
- Conditioned Medium Preparation (Growth Factor/Cytokine)
- Dual Immunofluorescence Cell Staining
- Flow Cytometry prep & labeling
- Immunoprecipitation Guide
- Immunohistochemistry Paraffin
- Immunofluorescence Cell Staining
- In Vitro Kinase Assay
- Isolate Leukocytes from whole blood
- Lentivirus Technology
- Membrane Stripping and Reprobing
- Nested RT-PCR
- Nuclear Extract Preparation
- Puromycin Selection for shRNA
- shRNA Transfection
- siRNA Transfection
- Sodium Orthovanadate Activation
- Western Blotting Guide
- Western Blot Optimization
- Zinc Fixation