User:Korey Griffin: Difference between revisions
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==Contact | ==Contact== | ||
*Korey Griffin | [[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]] | ||
[[Image:halfdome.jpg|thumb|right|Half Dome]] | |||
*Korey Griffin MS | |||
*Santa Cruz Biotechnology, Inc. | *Santa Cruz Biotechnology, Inc. | ||
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]] | *[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]] | ||
Bench research generates advancements in knowledge & health. Reagent quality & protocol awareness are essential for success in molecular & cell biology. Communication on reagent quality & procedure optimization facilitates successful R&D efforts. | |||
Experiments (ie immuno (western) blotting) contain many important stages & considerations. Below are a valuable collection of resources, that will improve awareness & success. | |||
==Methods & Techniques== | |||
[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]] | |||
[[Image:Kingscanyon.jpg|thumb|right|Kings Canyon]] | |||
[[Image:Nisenemarks.jpg|thumb|right|Sand Point]] | |||
*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics] | |||
*[http://openwetware.org/wiki/Griffin:Antibody_Peptide_Neutralization Antibody Peptide Neutralization] | |||
*[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios Antibody Protein Coupling (F:P) Ratios] | |||
*[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Antibody Elution Buffers] | |||
*[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Antibody Related Solutions & Recipes] | |||
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique] | |||
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation] | |||
*[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium Preparation (Growth Factor/Cytokine)] | |||
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining] | |||
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling] | |||
*[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide Immunoprecipitation Guide] | |||
*[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin Immunohistochemistry Paraffin] | |||
*[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining Immunofluorescence Cell Staining] | |||
*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay] | |||
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood] | |||
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology] | |||
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing] | |||
*[http://openwetware.org/wiki/Griffin:Nested_RT-PCR Nested RT-PCR] | |||
*[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extract Preparation] | |||
*[http://openwetware.org/wiki/Griffin:Puromycin_Selection Puromycin Selection for shRNA] | |||
*[http://openwetware.org/wiki/Griffin:shRNA_Transfection shRNA Transfection] | |||
*[http://openwetware.org/wiki/Griffin:siRNA_transfection siRNA Transfection] | |||
*[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Sodium Orthovanadate Activation] | |||
*[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide] | |||
*[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization] | |||
*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation] | |||
==Links== | |||
*[http://www.atcc.org ATCC Cell Lines] | |||
*[http://www.antibodybeyond.com/ Organelle and Lineage Markers] | |||
*[http://safety.science.tamu.edu/dot.html DOT guidelines] | |||
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table] | |||
*[http://www.proteinatlas.org/ Human Protein Atlas] | |||
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm] | |||
[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]] | |||
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]] | |||
Revision as of 11:56, 9 October 2014
Contact
- Korey Griffin MS
- Santa Cruz Biotechnology, Inc.
- Email me through OpenWetWare
Bench research generates advancements in knowledge & health. Reagent quality & protocol awareness are essential for success in molecular & cell biology. Communication on reagent quality & procedure optimization facilitates successful R&D efforts.
Experiments (ie immuno (western) blotting) contain many important stages & considerations. Below are a valuable collection of resources, that will improve awareness & success.
Methods & Techniques
- Antibody Basics
- Antibody Peptide Neutralization
- Antibody Protein Coupling (F:P) Ratios
- Antibody Elution Buffers
- Antibody Related Solutions & Recipes
- Antigen Retrieval Technique
- Chromatin Immunoprecipitation
- Conditioned Medium Preparation (Growth Factor/Cytokine)
- Dual Immunofluorescence Cell Staining
- Flow Cytometry prep & labeling
- Immunoprecipitation Guide
- Immunohistochemistry Paraffin
- Immunofluorescence Cell Staining
- In Vitro Kinase Assay
- Isolate Leukocytes from whole blood
- Lentivirus Technology
- Membrane Stripping and Reprobing
- Nested RT-PCR
- Nuclear Extract Preparation
- Puromycin Selection for shRNA
- shRNA Transfection
- siRNA Transfection
- Sodium Orthovanadate Activation
- Western Blotting Guide
- Western Blot Optimization
- Zinc Fixation
Links
- ATCC Cell Lines
- Organelle and Lineage Markers
- DOT guidelines
- Fluorochrome Table
- Human Protein Atlas
- Oligo Tm
