User:Korey Griffin: Difference between revisions

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==Contact Info==
==Contact==
[[Image:image1.jpg|thumb|right|Pondering the nuances of protocol optimization]]
 
[[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]
 
[[Image:halfdome.jpg|thumb|right|Half Dome]]


*Korey Griffin
*Korey Griffin
*Santa Cruz Biotechnology, Inc.
*Santa Cruz Biotechnology, Inc.
*2145 Delaware Avenue Santa Cruz, CA 95060
*Santa Cruz, California, USA
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]


I am a sales and technical support representative for [http://www.scbt.com/ Santa Cruz Biotechnology, Inc]. My primary focus is building relationships with industry and academic institutions extending beyond the products themselves. Researcher time and labor at the bench is very important to consider when running experiments over time. I am joining OWW in order to contribute research antibody (immunoglobulin) and siRNA/shRNA related protocols in order to facilitate the research experience. When I am not at pondering the nuances of protocol optimization, I enjoy surfing, mt. biking, and good margaritas.  
Bench research generates advancements in knowledge & health. Reagent quality & protocol awareness are essential for success in molecular & cell biology. Communication on reagent quality & procedure optimization facilitates successful R&D efforts.


Value your time, know the reagents & have confidence in your technique.
Experiments (ie immuno (western) blotting) contain many important stages & considerations. Below are a valuable collection of resources, that will improve awareness & success.


Please feel free to call or email me at any time regarding Antibody/RNAi related questions or Issues.
==Methods & Techniques==


==Education==
[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]]
<!--Include info about your educational background-->


* 2000, MS Biochemistry, University of Missouri-Columbia
[[Image:Kingscanyon.jpg|thumb|right|Kings Canyon]]
Advisor: [http://biochem.missouri.edu/gweisman.php Gary Weisman Ph.D.]


* 1997, BS, University of Missouri-Columbia
[[Image:Nisenemarks.jpg|thumb|right|Sand Point]]


==Research interests==
*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics]
<!-- Feel free to add brief descriptions to your research interests as well -->
*[http://openwetware.org/wiki/Griffin:Antibody_Peptide_Neutralization Antibody Peptide Neutralization]
# Communication between antibody/reagent vendors and bench scientists
*[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios Antibody Protein Coupling (F:P) Ratios]
# Antibody protocol optimization and imaging
*[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Antibody Elution Buffers]
# siRNA & shRNA design & experimental methodology
*[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Antibody Related Solutions & Recipes]
# Antibody design & experimental methodology
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
 
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
==Publications==
*[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium Preparation (Growth Factor/Cytokine)]
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications. You can add or remove lines as needed -->
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
<biblio>
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
#Paper1 pmid=14670955
#Paper2 pmid=11331301
</biblio>
 
==Useful links==
*[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide  Immunoprecipitation Guide]
*[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide  Immunoprecipitation Guide]
*[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin Immunohistochemistry Paraffin]
*[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
*[http://openwetware.org/wiki/Griffin:Nested_RT-PCR Nested RT-PCR]
*[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extract Preparation]
*[http://openwetware.org/wiki/Griffin:Puromycin_Selection Puromycin Selection for shRNA]
*[http://openwetware.org/wiki/Griffin:shRNA_Transfection shRNA Transfection]
*[http://openwetware.org/wiki/Griffin:siRNA_transfection siRNA Transfection]
*[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Sodium Orthovanadate Activation]
*[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide]
*[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide]
*[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization]
*[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization]
*[http://www.openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extract Preparation]
*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]
*[http://www.openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
 
*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics]
==Links==
 
*[http://www.atcc.org ATCC Cell Lines]
*[http://www.antibodybeyond.com/ Organelle and Lineage Markers]
*[http://safety.science.tamu.edu/dot.html DOT guidelines]
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
*[http://www.proteinatlas.org/ Human Protein Atlas]
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm]
 
[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]


*[[OpenWetWare:Welcome|Introductory tutorial]]
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
*[[Help|OpenWetWare help pages]]

Revision as of 11:53, 10 December 2014

Contact

Santa Cruz West cliff
Half Dome

Bench research generates advancements in knowledge & health. Reagent quality & protocol awareness are essential for success in molecular & cell biology. Communication on reagent quality & procedure optimization facilitates successful R&D efforts.

Experiments (ie immuno (western) blotting) contain many important stages & considerations. Below are a valuable collection of resources, that will improve awareness & success.

Methods & Techniques

Santa Cruz Mountains
Kings Canyon
Sand Point

Links

North County Santa Cruz
Liberty Cap & Nevada Falls
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