User:Korey Griffin: Difference between revisions

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==Contact Info==
==Contact==
[[Image:image1.jpg|thumb|right|Pondering the nuances of protocol optimization]]
 
[[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]
 
[[Image:halfdome.jpg|thumb|right|Half Dome]]


*Korey Griffin
*Korey Griffin
*Santa Cruz Biotechnology, Inc.
*Santa Cruz Biotechnology, Inc.
*2145 Delaware Avenue Santa Cruz, CA 95060
*Santa Cruz, California, USA
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]


I am a technical support representative for [http://www.scbt.com/ Santa Cruz Biotechnology, Inc]. My primary focus is building relationships with industry and academic institutions for anyone from graduate students to post-doctoral researchers alike, who are running new experiments for the first time or trying out new antibodies.  
Bench research generates advancements in knowledge & health. Reagent quality & protocol awareness are essential for success in molecular & cell biology. Communication on reagent quality & procedure optimization facilitates successful R&D efforts.


Experienced technicians in all aspects of research become so skilled at bench techniques that the nuances of the procedures become second nature, and often times these nuances influence the quality and consistency of obtaining good data
Experiments (ie immuno (western) blotting) contain many important stages & considerations. Below are a valuable collection of resources, that will improve awareness & success.


I seek to create new ways for bench scientists to optimize their protocols so that results are more purposeful toward producing publication quality data. Researcher time and labor at the bench is very important to consider when running experiments over time. I am joining OWW in order to contribute research antibody (immunoglobulin) and siRNA/shRNA related protocols in order to facilitate the research experience. When I am not at pondering the nuances of protocol optimization (check useful links below, ie [http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization]), I enjoy surfing, mt. biking, camping, and pen&ink artwork.
==Methods & Techniques==


Value your time, know the reagents & have confidence in your technique.
[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]]


I wish you success!
[[Image:Kingscanyon.jpg|thumb|right|Kings Canyon]]


==Education==
[[Image:Nisenemarks.jpg|thumb|right|Sand Point]]
<!--Include info about your educational background-->


* 2000, MS Biochemistry, University of Missouri-Columbia
*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics]
Advisor: [http://biochem.missouri.edu/gweisman.php Gary Weisman Ph.D.]
*[http://openwetware.org/wiki/Griffin:Antibody_Peptide_Neutralization Antibody Peptide Neutralization]
*[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios Antibody Protein Coupling (F:P) Ratios]
*[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Antibody Elution Buffers]
*[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Antibody Related Solutions & Recipes]
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
*[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium Preparation (Growth Factor/Cytokine)]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
*[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide  Immunoprecipitation Guide]
*[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin Immunohistochemistry Paraffin]
*[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
*[http://openwetware.org/wiki/Griffin:Nested_RT-PCR Nested RT-PCR]
*[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extract Preparation]
*[http://openwetware.org/wiki/Griffin:Puromycin_Selection Puromycin Selection for shRNA]
*[http://openwetware.org/wiki/Griffin:shRNA_Transfection shRNA Transfection]
*[http://openwetware.org/wiki/Griffin:siRNA_transfection siRNA Transfection]
*[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Sodium Orthovanadate Activation]
*[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide]
*[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization]
*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]


* 1997, BS, University of Missouri-Columbia
==Links==


==Research interests==
*[http://www.atcc.org ATCC Cell Lines]
<!-- Feel free to add brief descriptions to your research interests as well -->
*[http://www.antibodybeyond.com/ Organelle and Lineage Markers]
# Communication between antibody/reagent vendors and bench scientists
*[http://safety.science.tamu.edu/dot.html DOT guidelines]
# Antibody protocol optimization and imaging
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
# siRNA & shRNA design & experimental methodology
*[http://www.proteinatlas.org/ Human Protein Atlas]
# Antibody design & experimental methodology
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm]


==Publications==
[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications.  You can add or remove lines as needed -->
<biblio>
#Paper1 pmid=14670955
#Paper2 pmid=11331301
</biblio>
 
==Useful links==
*[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide  Immunoprecipitation Guide]
*[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide]
*[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization]
*[http://www.openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extract Preparation]
*[http://www.openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin Immunohistochemistry Paraffin]
*[http://www.openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://www.openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
*[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Sodium Orthovanadate Activation]
*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics]


*[[OpenWetWare:Welcome|Introductory tutorial]]
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
*[[Help|OpenWetWare help pages]]

Revision as of 11:53, 10 December 2014

Contact

Santa Cruz West cliff
Half Dome

Bench research generates advancements in knowledge & health. Reagent quality & protocol awareness are essential for success in molecular & cell biology. Communication on reagent quality & procedure optimization facilitates successful R&D efforts.

Experiments (ie immuno (western) blotting) contain many important stages & considerations. Below are a valuable collection of resources, that will improve awareness & success.

Methods & Techniques

Santa Cruz Mountains
Kings Canyon
Sand Point

Links

North County Santa Cruz
Liberty Cap & Nevada Falls
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