User:Korey Griffin: Difference between revisions

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==Contact Info==
==Contact==


[[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]
[[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]
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*Korey Griffin
*Korey Griffin
*Santa Cruz Biotechnology, Inc.
*Santa Cruz Biotechnology, Inc.
*2145 Delaware Avenue Santa Cruz, CA 95060
*Santa Cruz, California, USA
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]


I am currently a technical support representative for [http://www.scbt.com/ Santa Cruz Biotechnology, Inc]. My primary focus is communication with researchers who are coordinating chemical, Immuno- & RNAi experiments with SCBT reagents.  
Bench research generates advancements in knowledge & health. Reagent quality & protocol awareness are essential for success in molecular & cell biology. Communication on reagent quality & procedure optimization facilitates successful R&D efforts.


Molecular/cell biologists eventually through the test of reproducing data become so skillful at bench techniques, that the nuances of these procedures become second nature. Often times these nuances are what determine the overall quality and consistency of experimental results. Like crafting a quality beer, premium ingredients are essential, although the experience and intuition of the brewmaster determines the quality of the result.
Experiments (ie immuno (western) blotting) contain many important stages & considerations. Below are a valuable collection of resources, that will improve awareness & success.


Researcher time and labor spent at the bench is very important in every way; results are good and they take time. With so may variables associated with each molecular/cell protocol, even the most simple of procedures contains an extraordinary variable set. I am contributing research antibody (immunoglobulin) and siRNA/shRNA related information in order to increase awareness for scientists and to optimize the research experience.
==Methods & Techniques==
 
==Methods & Techniques for Immunochemists==


[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]]
[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]]
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*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
*[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium Preparation (Growth Factor/Cytokine)]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
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*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
*[http://openwetware.org/wiki/Isolate_leydig_cells_from_testes Isolate Leydig cells]
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
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*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]
*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]


==Useful Links==
==Links==


[[Image:Sunset.jpg|thumb|right|Santa Cruz Sunset]]
*[http://www.atcc.org ATCC Cell Lines]
 
*[http://www.antibodybeyond.com/ Organelle and Lineage Markers]
*[http://www.atcc.org Cell Lines]
*[http://www.antibodybeyond.com/ Cell Marker]
*[http://safety.science.tamu.edu/dot.html DOT guidelines]
*[http://safety.science.tamu.edu/dot.html DOT guidelines]
*[http://www.exactantigen.com/ ExactAntigen]
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
*[http://www.dsi.univ-paris5.fr/genatlas/ Gene Atlas]
*[http://www.proteinatlas.org/ Human Protein Atlas]
*[http://www.proteinatlas.org/ Human Protein Atlas]
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm]
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm]
==Experience==


[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]
[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]


*2007-2008: [http://saveourshores.org/ Save our Shores] Sanctuary Steward, Santa Cruz CA. Save Our Shores is a non-profit marine conservation organization in Santa Cruz, California whose mission is marine conservation through policy research, education, and citizen involvement.
*2005-2006: [http://www.rideawave.org/ Ride-A-Wave volunteer], Santa Cruz CA. RAW is a Santa Cruz based nonprofit org whose mission is to give children with special needs the chance to feel the thrill of riding a wave and experience a safe, fun-filled day at the beach, whether they are physically, developmentally or economically challenged.
*1997: Summer Youth Camp Counselor for [http://www.navigators.org/us/ministries/eaglelake/ Eagle Lake Camp], Pike Forest CO.
*1995,1996: Canoe Guide/Interpreter [http://www.ntier.org/ Charles L. Sommers Canoe Base], Ely MN: Groups are outfitted at the Base to travel by canoe through canoe country, the Boundary Waters Canoe Area Wilderness (BWCAW), Ontario's Quetico Provincial Park, and points beyond. The BWCAW and Quetico together form a roadless area of about a million acres with deep glacial lakes and densely forested cover.
==Education & Publication==
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
* 2000, MS Biochemistry, [http://www.missouri.edu/ University of Missouri-Columbia], Advisor: [http://biochem.missouri.edu/gweisman.php Gary Weisman Ph.D.]
* 1997, BS, University of Missouri-Columbia
<biblio>
#Paper1 pmid=14670955
#Paper2 pmid=11331301
</biblio>

Revision as of 11:53, 10 December 2014

Contact

Santa Cruz West cliff
Half Dome

Bench research generates advancements in knowledge & health. Reagent quality & protocol awareness are essential for success in molecular & cell biology. Communication on reagent quality & procedure optimization facilitates successful R&D efforts.

Experiments (ie immuno (western) blotting) contain many important stages & considerations. Below are a valuable collection of resources, that will improve awareness & success.

Methods & Techniques

Santa Cruz Mountains
Kings Canyon
Sand Point

Links

North County Santa Cruz
Liberty Cap & Nevada Falls
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