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| ==Contact Info== | | ==[https://www.researchgate.net/profile/Korey-Griffin Korey D Griffin]== |
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| [[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]
| | =Procedure= |
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| [[Image:halfdome.jpg|thumb|right|Half Dome]] | | *[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibodies] |
| | *[https://www.culturecollections.org.uk/products/celllines/index.aspx Cell Culture Collections] |
| | *[http://openwetware.org/wiki/Griffin:Antibody_Peptide_Neutralization Peptide Neutralization] |
| | *[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios (F:P) OD 260/280] |
| | *[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Elution Buffers] |
| | *[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Solutions] |
| | *[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval] |
| | *[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation ChIP] |
| | *[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium] |
| | *[http://openwetware.org/wiki/Griffin:CRISPR CRISPR/Cas] |
| | *[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining 2 Channel IF] |
| | *[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining IF Fixation] |
| | *[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Fluorphores ExEm] |
| | *[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide Protein Extraction] |
| | *[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin IHC] |
| | *[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay KA] |
| | *[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Leukocytes] |
| | *[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus] |
| | *[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Reprobing] |
| | *[http://openwetware.org/wiki/Griffin:Proteomics Proteomics] |
| | *[http://openwetware.org/wiki/Griffin:Nested_RT-PCR PCR] |
| | *[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extracts] |
| | *[http://openwetware.org/wiki/Griffin:Puromycin_Selection Puromycin] |
| | *[http://openwetware.org/wiki/Griffin:siRNA_transfection RNAi/siRNA] |
| | *[http://openwetware.org/wiki/Griffin:shRNA_Transfection DNA/RNA Delivery] |
| | *[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Na₃VO₄] |
| | *[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting WB] |
| | *[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization WB Optimize] |
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| *Korey Griffin M.S. | | ==Survey== |
| *Santa Cruz Biotechnology, Inc. | | *[https://www.ncbi.nlm.nih.gov/pubmed/ Pubmed] |
| *2145 Delaware Avenue Santa Cruz, CA 95060 | | *[https://chat.openai.com/auth/login GPT] |
| *800-457-3801 x129
| | *[http://openwetware.org/wiki/Griffin:Trending Trends] |
| *[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]] | | *[https://en.bio-protocol.org/bio101/Default.aspx Protocol] |
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| I am currently a technical representative for [http://www.scbt.com/ Santa Cruz Biotechnology, Inc] where communication with researchers in institutions who are coordinating chemical, immunoglobulin, & RNAi experiments with SCBT reagents and labware can benefit from real time support.
| | =Genomic= |
| | *[https://www.ncbi.nlm.nih.gov/gene Entrez] |
| | *[https://www.genecards.org/ Genecards] |
| | *[http://atlasgeneticsoncology.org Atlas Gene] |
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| Research time & bench labor is a valuable effort. To have a high degree of confidence in both the reagent quality, and the procedure can have further assurance of success through a vendor technical representative. The means to obtain, test, and provide feedback is beneficial.
| | =Molecular= |
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| There are several nuances and variables for each molecular/cell protocol that determines how the result turns out; apparent & straight-forward procedures (ie immuno (western) blotting) contain an extraordinary amount of important steps. Below are resources for research antibody and RNAi studies, that can improve your next experiment. I hope that you may find something useful to bring you success in your next experiment.
| | *[https://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST] |
| | *[https://www.idtdna.com/pages/tools/oligoanalyzer IDT Analyzer] |
| | *[http://www.oligoevaluator.com/LoginServlet Sigma OligoEvaluator(tm)] |
| | *[https://eurofinsgenomics.eu/en/ecom/tools/oligo-analysis/ Eurofins Genomics] |
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| ==Methods & Techniques for Immunochemists== | | =Cell= |
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| [[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]] | | *[http://www.atcc.org/ ATCC] |
| | *[https://www.cellosaurus.org/ SIB] |
| | *[http://www.antibodybeyond.com/ Markers] |
| | *[https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure.html Organelle Markers] |
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| [[Image:Kingscanyon.jpg|thumb|right|Kings Canyon]]
| | =Protein= |
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| [[Image:Nisenemarks.jpg|thumb|right|Sand Point]]
| | *[https://www.ebi.ac.uk/Tools/msa/clustalo/ Clustal Omega] |
| | | *[http://www.proteinatlas.org/ HPA] |
| *[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics]
| | *[https://www.uniprot.org/ UniProt] |
| *[http://openwetware.org/wiki/Griffin:Antibody_Peptide_Neutralization Antibody Peptide Neutralization]
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| *[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios Antibody Protein Coupling (F:P) Ratios]
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| *[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Antibody Elution Buffers]
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| *[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Antibody Related Solutions & Recipes]
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| *[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
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| *[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
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| *[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
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| *[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
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| *[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide Immunoprecipitation Guide]
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| *[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin Immunohistochemistry Paraffin]
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| *[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining Immunofluorescence Cell Staining]
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| *[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
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| *[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
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| *[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
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| *[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
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| *[http://openwetware.org/wiki/Griffin:Nested_RT-PCR Nested RT-PCR] | |
| *[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extract Preparation]
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| *[http://openwetware.org/wiki/Griffin:Puromycin_Selection Puromycin Selection for shRNA]
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| *[http://openwetware.org/wiki/Griffin:shRNA_Transfection shRNA Transfection]
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| *[http://openwetware.org/wiki/Griffin:siRNA_transfection siRNA Transfection]
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| *[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Sodium Orthovanadate Activation]
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| *[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide]
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| *[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization]
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| *[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]
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| ==Useful Links==
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| *[http://www.atcc.org Cell Lines]
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| *[http://www.antibodybeyond.com/ Cell Marker]
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| *[http://safety.science.tamu.edu/dot.html DOT guidelines]
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| *[http://www.exactantigen.com/ ExactAntigen]
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| *[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
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| *[http://www.dsi.univ-paris5.fr/genatlas/ Gene Atlas]
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| *[http://www.proteinatlas.org/ Human Protein Atlas] | |
| *[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm] | |
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| ==Experience==
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| [[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]
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| *2007-2009: [http://saveourshores.org/ Save our Shores] Sanctuary Steward, Santa Cruz CA. Save Our Shores is a non-profit marine conservation organization in Santa Cruz, California whose mission is marine conservation through policy research, education, and citizen involvement.
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| *2005-2006: [http://www.rideawave.org/ Ride-A-Wave volunteer], Santa Cruz CA. RAW is a Santa Cruz based nonprofit org whose mission is to give children with special needs the chance to feel the thrill of riding a wave and experience a safe, fun-filled day at the beach, whether they are physically, developmentally or economically challenged.
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| *1997: Summer Youth Camp Counselor for [http://www.navigators.org/us/ministries/eaglelake/ Eagle Lake Camp], Pike Forest CO.
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| *1995,1996: Canoe Guide/Interpreter [http://www.ntier.org/ Charles L. Sommers Canoe Base], Ely MN: Groups are outfitted at the Base to travel by canoe through canoe country, the Boundary Waters Canoe Area Wilderness (BWCAW), Ontario's Quetico Provincial Park, and points beyond. The BWCAW and Quetico together form a roadless area of about a million acres with deep glacial lakes and densely forested cover.
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| ==Education & Publication==
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| [[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
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| * 2000, MS Biochemistry, [http://www.missouri.edu/ University of Missouri-Columbia], Advisor: [http://biochem.missouri.edu/gweisman.php Gary Weisman Ph.D.]
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| * 1997, BS, University of Missouri-Columbia
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| ==Publications==
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| J Biol Chem. 2004 Feb 27;279(9):8212-8. Epub 2003 Dec 11. Src homology 3 binding sites in the P2Y2 nucleotide receptor interact with Src and regulate activities of Src, proline-rich tyrosine kinase 2, and growth factor receptors. Liu J, Liao Z, Camden J, Griffin KD, Garrad RC, Santiago-Pérez LI, González FA, Seye CI, Weisman GA, Erb L. PMID: 14670955
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| J Cell Biol. 2001 Apr 30;153(3):491-501. An RGD sequence in the P2Y(2) receptor interacts with alpha(V)beta(3) integrins and is required for G(o)-mediated signal transduction. Erb L, Liu J, Ockerhausen J, Kong Q, Garrad RC, Griffin K, Neal C, Krugh B, Santiago-Pérez LI, González FA, Gresham HD, Turner JT, Weisman GA. PMID: 11331301
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