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| ==Contact== | | ==[https://www.researchgate.net/profile/Korey-Griffin Korey D Griffin]== |
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| [[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]
| | =Procedure= |
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| [[Image:halfdome.jpg|thumb|right|Half Dome]] | | *[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibodies] |
| | *[https://www.culturecollections.org.uk/products/celllines/index.aspx Cell Culture Collections] |
| | *[http://openwetware.org/wiki/Griffin:Antibody_Peptide_Neutralization Peptide Neutralization] |
| | *[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios (F:P) OD 260/280] |
| | *[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Elution Buffers] |
| | *[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Solutions] |
| | *[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval] |
| | *[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation ChIP] |
| | *[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium] |
| | *[http://openwetware.org/wiki/Griffin:CRISPR CRISPR/Cas] |
| | *[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining 2 Channel IF] |
| | *[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining IF Fixation] |
| | *[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Fluorphores ExEm] |
| | *[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide Protein Extraction] |
| | *[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin IHC] |
| | *[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay KA] |
| | *[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Leukocytes] |
| | *[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus] |
| | *[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Reprobing] |
| | *[http://openwetware.org/wiki/Griffin:Proteomics Proteomics] |
| | *[http://openwetware.org/wiki/Griffin:Nested_RT-PCR PCR] |
| | *[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extracts] |
| | *[http://openwetware.org/wiki/Griffin:Puromycin_Selection Puromycin] |
| | *[http://openwetware.org/wiki/Griffin:siRNA_transfection RNAi/siRNA] |
| | *[http://openwetware.org/wiki/Griffin:shRNA_Transfection DNA/RNA Delivery] |
| | *[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Na₃VO₄] |
| | *[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting WB] |
| | *[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization WB Optimize] |
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| *Korey Griffin MS | | ==Survey== |
| *Santa Cruz Biotechnology, Inc. | | *[https://www.ncbi.nlm.nih.gov/pubmed/ Pubmed] |
| *[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]] | | *[https://chat.openai.com/auth/login GPT] |
| | *[http://openwetware.org/wiki/Griffin:Trending Trends] |
| | *[https://en.bio-protocol.org/bio101/Default.aspx Protocol] |
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| I am a technical representative at [http://www.scbt.com/ Santa Cruz Biotechnology, Inc] where I communicate with researchers coordinating biochemical, immunoglobulin, & RNAi studies.
| | =Genomic= |
| | *[https://www.ncbi.nlm.nih.gov/gene Entrez] |
| | *[https://www.genecards.org/ Genecards] |
| | *[http://atlasgeneticsoncology.org Atlas Gene] |
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| Bench research is valuable to human health and awareness. Having confidence in reagent quality & protocol is essential to success in molecular/cell biology. My goal is to facilitate successful research with SCBT reagents through 2-way communication with clients on product quality & procedure.
| | =Molecular= |
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| Hundreds of variables in molecular/cell protocols determine reliable data. Apparent & straight-forward procedures (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of links to resources for research antibody and RNAi studies, that can improve awareness & success.
| | *[https://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST] |
| | *[https://www.idtdna.com/pages/tools/oligoanalyzer IDT Analyzer] |
| | *[http://www.oligoevaluator.com/LoginServlet Sigma OligoEvaluator(tm)] |
| | *[https://eurofinsgenomics.eu/en/ecom/tools/oligo-analysis/ Eurofins Genomics] |
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| ==Methods & Techniques== | | =Cell= |
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| [[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]] | | *[http://www.atcc.org/ ATCC] |
| | *[https://www.cellosaurus.org/ SIB] |
| | *[http://www.antibodybeyond.com/ Markers] |
| | *[https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure.html Organelle Markers] |
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| [[Image:Kingscanyon.jpg|thumb|right|Kings Canyon]]
| | =Protein= |
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| [[Image:Nisenemarks.jpg|thumb|right|Sand Point]]
| | *[https://www.ebi.ac.uk/Tools/msa/clustalo/ Clustal Omega] |
| | | *[http://www.proteinatlas.org/ HPA] |
| *[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics]
| | *[https://www.uniprot.org/ UniProt] |
| *[http://openwetware.org/wiki/Griffin:Antibody_Peptide_Neutralization Antibody Peptide Neutralization]
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| *[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios Antibody Protein Coupling (F:P) Ratios]
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| *[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Antibody Elution Buffers]
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| *[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Antibody Related Solutions & Recipes]
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| *[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique] | |
| *[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
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| *[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
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| *[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
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| *[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide Immunoprecipitation Guide]
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| *[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin Immunohistochemistry Paraffin]
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| *[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining Immunofluorescence Cell Staining]
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| *[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
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| *[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
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| *[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
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| *[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
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| *[http://openwetware.org/wiki/Griffin:Nested_RT-PCR Nested RT-PCR]
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| *[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extract Preparation]
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| *[http://openwetware.org/wiki/Griffin:Puromycin_Selection Puromycin Selection for shRNA]
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| *[http://openwetware.org/wiki/Griffin:shRNA_Transfection shRNA Transfection]
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| *[http://openwetware.org/wiki/Griffin:siRNA_transfection siRNA Transfection]
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| *[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Sodium Orthovanadate Activation]
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| *[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide]
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| *[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization]
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| *[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]
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| ==Links==
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| *[http://www.atcc.org ATCC Cell Lines]
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| *[http://www.antibodybeyond.com/ Organelle and Lineage Markers]
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| *[http://safety.science.tamu.edu/dot.html DOT guidelines]
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| *[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
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| *[http://www.proteinatlas.org/ Human Protein Atlas] | |
| *[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm] | |
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| ==Experience==
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| [[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]
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| [[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
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| *2009-2012: CTO [http://slatsmanduapparel.com/ Slatsmandu USA], Santa Cruz CA. Slatsmandu USA creates utility belts with integrated advanced features.
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| *2007-2012: Volunteer [http://saveourshores.org/ Save our Shores], Santa Cruz CA. Save Our Shores is a non-profit marine conservation organization in Santa Cruz, California whose mission is marine conservation through policy research, education, and citizen involvement.
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| *2005-2006: Volunteer [http://www.rideawave.org/ Ride-A-Wave volunteer], Santa Cruz CA. RAW is a Santa Cruz based nonprofit org whose mission is to give children with special needs the chance to feel the thrill of riding a wave and experience a safe, fun-filled day at the beach.
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| *2000, MS Biochemistry, [http://www.missouri.edu/ University of Missouri-Columbia]
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| *1997: Counselor [http://www.navigators.org/us/ministries/eaglelake/ Eagle Lake Camp], Pike Forest CO.
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| *1995,1996: Interpreter [http://www.ntier.org/ Charles L. Sommers Canoe Base], Ely MN: Boundary Waters Canoe Area Wilderness (BWCAW) and Quetico together form a roadless area ~1 million acres.
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