User:Korey Griffin: Difference between revisions

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==Contact==
==[https://www.researchgate.net/profile/Korey-Griffin Korey D Griffin]==


[[Image:Santa Cruz.jpg|thumb|right|Santa Cruz West cliff]]
=Procedure=


[[Image:halfdome.jpg|thumb|right|Half Dome]]
*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibodies]
*[https://www.culturecollections.org.uk/products/celllines/index.aspx Cell Culture Collections]
*[http://openwetware.org/wiki/Griffin:Antibody_Peptide_Neutralization Peptide Neutralization]
*[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios (F:P) OD 260/280]
*[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Elution Buffers]
*[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Solutions]
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval]
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation ChIP]
*[http://openwetware.org/wiki/Griffin:Conditioned_Medium_Preparation Conditioned Medium]
*[http://openwetware.org/wiki/Griffin:CRISPR CRISPR/Cas]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining 2 Channel IF]
*[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining IF Fixation]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Fluorphores ExEm]
*[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide Protein Extraction]
*[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin IHC]
*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay KA]
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Leukocytes]
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Reprobing]
*[http://openwetware.org/wiki/Griffin:Proteomics Proteomics]
*[http://openwetware.org/wiki/Griffin:Nested_RT-PCR PCR]
*[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extracts]
*[http://openwetware.org/wiki/Griffin:Puromycin_Selection Puromycin]
*[http://openwetware.org/wiki/Griffin:siRNA_transfection RNAi/siRNA]
*[http://openwetware.org/wiki/Griffin:shRNA_Transfection DNA/RNA Delivery]
*[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Na₃VO₄]
*[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting WB]
*[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization WB Optimize]


*Korey Griffin MS
==Survey==
*Santa Cruz Biotechnology, Inc.
*[https://www.ncbi.nlm.nih.gov/pubmed/ Pubmed]
*[[Special:Emailuser/Korey Griffin|Email me through OpenWetWare]]
*[https://chat.openai.com/auth/login GPT]
*[http://openwetware.org/wiki/Griffin:Trending Trends]
*[https://en.bio-protocol.org/bio101/Default.aspx Protocol]


Bench research leads to advancements in human health and awareness. Confidence in reagent quality & protocol is essential to success in molecular & cell biology. I facilitate scientists in creating successful R&D efforts in every phase; through 2-way communication on reagent quality & procedure optimization.
=Genomic=
*[https://www.ncbi.nlm.nih.gov/gene Entrez]
*[https://www.genecards.org/ Genecards]
*[http://atlasgeneticsoncology.org Atlas Gene]


Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward procedures (ie immuno (western) blotting) contain many important stages for consideration. Below are a collection of resources for Immunoglobulin and RNAi studies, that improve awareness & success.
=Molecular=


==Methods & Techniques==
*[https://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST]
*[https://www.idtdna.com/pages/tools/oligoanalyzer IDT Analyzer]
*[http://www.oligoevaluator.com/LoginServlet Sigma OligoEvaluator(tm)]
*[https://eurofinsgenomics.eu/en/ecom/tools/oligo-analysis/ Eurofins Genomics]


[[Image:SCmountains.jpg|thumb|right|Santa Cruz Mountains]]
=Cell=


[[Image:Kingscanyon.jpg|thumb|right|Kings Canyon]]
*[http://www.atcc.org/ ATCC]
*[https://www.cellosaurus.org/ SIB]
*[http://www.antibodybeyond.com/ Markers]
*[https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure.html Organelle Markers]


[[Image:Nisenemarks.jpg|thumb|right|Sand Point]]
=Protein=


*[http://openwetware.org/wiki/Griffin:Antibody_Basics Antibody Basics]
*[https://www.ebi.ac.uk/Tools/msa/clustalo/ Clustal Omega]
*[http://openwetware.org/wiki/Griffin:Antibody_Peptide_Neutralization Antibody Peptide Neutralization]
*[http://www.proteinatlas.org/ HPA]
*[http://openwetware.org/wiki/Griffin:Antibody_Protein_Coupling_%28F:P%29_Ratios Antibody Protein Coupling (F:P) Ratios]
*[https://www.uniprot.org/ UniProt]
*[http://openwetware.org/wiki/Griffin:Antibody_Elution_Buffers Antibody Elution Buffers]
*[http://openwetware.org/wiki/Griffin:Antibody_Related_Solutions_%26_Recipes Antibody Related Solutions & Recipes]
*[http://openwetware.org/wiki/Griffin:Antigen_Retrieval_Technique Antigen Retrieval Technique]
*[http://openwetware.org/wiki/Griffin:Chromatin_Immunoprecipitation Chromatin Immunoprecipitation]
*[http://openwetware.org/wiki/Griffin:Dual_Immunofluorescence_Cell_Staining Dual Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:Flow_Cytometry_prep_%26_labeling Flow Cytometry prep & labeling]
*[http://openwetware.org/wiki/Griffin:_Ultimate_Immunoprecipitation_Guide  Immunoprecipitation Guide]
*[http://openwetware.org/wiki/Griffin:Immunohistochemistry_Paraffin Immunohistochemistry Paraffin]
*[http://openwetware.org/wiki/Griffin:Immunofluorescence_Cell_Staining Immunofluorescence Cell Staining]
*[http://openwetware.org/wiki/Griffin:In_Vitro_Kinase_Assay In Vitro Kinase Assay]
*[http://openwetware.org/wiki/Griffin:Isolate_Leukocytes_from_whole_blood Isolate Leukocytes from whole blood]
*[http://openwetware.org/wiki/Griffin:Lentivirus_Technology Lentivirus Technology]
*[http://openwetware.org/wiki/Griffin:Membrane_Stripping_and_Reprobing Membrane Stripping and Reprobing]
*[http://openwetware.org/wiki/Griffin:Nested_RT-PCR Nested RT-PCR]
*[http://openwetware.org/wiki/Griffin:Nuclear_Extract_Preparation Nuclear Extract Preparation]
*[http://openwetware.org/wiki/Griffin:Puromycin_Selection Puromycin Selection for shRNA]
*[http://openwetware.org/wiki/Griffin:shRNA_Transfection shRNA Transfection]
*[http://openwetware.org/wiki/Griffin:siRNA_transfection siRNA Transfection]
*[http://openwetware.org/wiki/Griffin:Sodium_Orthovanadate_Activation Sodium Orthovanadate Activation]
*[http://openwetware.org/wiki/Western_%28Immuno%29_Blotting Western Blotting Guide]
*[http://openwetware.org/wiki/Griffin:Western_Blot_Optimization Western Blot Optimization]
*[http://openwetware.org/wiki/Griffin:Zinc-Fixation Zinc Fixation]
 
==Links==
 
*[http://www.atcc.org ATCC Cell Lines]
*[http://www.antibodybeyond.com/ Organelle and Lineage Markers]
*[http://safety.science.tamu.edu/dot.html DOT guidelines]
*[http://flowcyt.salk.edu/fluo.html Fluorochrome Table]
*[http://www.proteinatlas.org/ Human Protein Atlas]
*[http://www.biophp.org/minitools/melting_temperature/demo.php Oligo Tm]
 
==Experience==
 
[[Image:northcounty.jpg|thumb|right|North County Santa Cruz]]
 
[[Image:Vernal Falls.jpg|thumb|right|Liberty Cap & Nevada Falls]]
 
*2007-2012: Volunteer [http://saveourshores.org/ Save our Shores], Santa Cruz CA. Save Our Shores is a non-profit marine conservation organization in Santa Cruz, California whose mission is marine conservation through policy research, education, and citizen involvement.
*2005-2006: Volunteer [http://www.rideawave.org/ Ride-A-Wave volunteer], Santa Cruz CA. RAW is a Santa Cruz based nonprofit org whose mission is to give children with special needs the chance to feel the thrill of riding a wave and experience a safe, fun-filled day at the beach.
*2000, MS Biochemistry, [http://www.missouri.edu/ University of Missouri-Columbia]
J Biol Chem. 2004 Feb 27;279(9):8212-8 / J Cell Biol. 2001 Apr 30;153(3):491-501

Latest revision as of 15:20, 11 October 2023

Korey D Griffin

Procedure

Survey

Genomic

Molecular

Cell

Protein

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