User:Korey Griffin
Contact Info
- Korey Griffin
- Santa Cruz Biotechnology, Inc.
- 2145 Delaware Avenue Santa Cruz, CA 95060
- Santa Cruz, California, USA
- Email me through OpenWetWare
I am currently a technical support representative for Santa Cruz Biotechnology, Inc. My primary focus is in communication with researchers who are coordinating Immunoglobulin-based and/or RNAi experiments with SCBT reagents.
Experienced scientists in molecular/cell biology research eventually through the test of reproducing data become so skillful at bench techniques and protocols, that the nuances of these procedures become second nature, and often times these nuances are what determine the overall quality and consistency of experimental results. Like preparing a gourmet recipe, premium ingredients are essential, though it is the experience of a master chef who determines the quality of the dish.
There are emerging ways for bench scientists to optimize their protocols so that results are more meaningful toward the ultimate goal of publishing quality data and expanding on our classical understanding of biological systems. The intrinsic value of time and labor spent at the bench is very important to consider when running molecular/cell experiments since the margin for obtaining a conclusive result is inverse to the number of variables associated with the protocol. And even the most simple of procedures contains an extraordinary variable set. I am contributing research antibody (immunoglobulin) and siRNA/shRNA related protocols in order to increase awareness for scientists and to optimize the research experience.
Methods & Techniques for Immunochemists
- Antibody Basics
- Antibody Peptide Neutralization
- Antibody Protein Coupling (F:P) Ratios
- Antibody Elution Buffers
- Antibody Related Solutions & Recipes
- Antigen Retrieval Technique
- Chromatin Immunoprecipitation
- Dual Immunofluorescence Cell Staining
- Flow Cytometry prep & labeling
- Immunoprecipitation Guide
- Immunohistochemistry Paraffin
- Immunofluorescence Cell Staining
- In Vitro Kinase Assay
- Isolate Leukocytes from whole blood
- Isolate Leydig cells
- Lentivirus Technology
- Membrane Stripping and Reprobing
- Nested RT-PCR
- Nuclear Extract Preparation
- Puromycin Selection for shRNA
- shRNA Transfection
- siRNA Transfection
- Sodium Orthovanadate Activation
- Western Blotting Guide
- Western Blot Optimization
- Zinc Fixation
Useful Links
Life
- 2007-2008: Save our Shores Sanctuary Steward, Santa Cruz CA. Save Our Shores is a non-profit marine conservation organization in Santa Cruz, California whose mission is marine conservation through policy research, education, and citizen involvement.
- 2006-2007: At-home tutoring for Math, Biology & Chemistry, Santa Cruz CA.
- 2005-2006: Ride-A-Wave volunteer, Santa Cruz CA. RAW is a Santa Cruz based nonprofit org whose mission is to give children with special needs the chance to feel the thrill of riding a wave and experience a safe, fun-filled day at the beach, whether they are physically, developmentally or economically challenged.
- 1997: Summer Youth Camp Counselor for Eagle Lake Camp, Pike Forest CO.
- 1995,1996: Canoe Guide/Interpreter Charles L. Sommers Canoe Base, Ely MN: Groups are outfitted at the Base to travel by canoe through canoe country, the Boundary Waters Canoe Area Wilderness (BWCAW), Ontario's Quetico Provincial Park, and points beyond. The BWCAW and Quetico together form a roadless area of about a million acres with deep glacial lakes and densely forested cover.
- 1993-1997: Sigma Chi, Xi Xi chapter.
- 1990: Eagle Scout, Boy Scouts of America.
Education
- 2000, MS Biochemistry, University of Missouri-Columbia, Advisor: Gary Weisman Ph.D.
- 1997, BS, University of Missouri-Columbia
Publications
- Liu J, Liao Z, Camden J, Griffin KD, Garrad RC, Santiago-Pérez LI, González FA, Seye CI, Weisman GA, and Erb L. Src homology 3 binding sites in the P2Y2 nucleotide receptor interact with Src and regulate activities of Src, proline-rich tyrosine kinase 2, and growth factor receptors. J Biol Chem. 2004 Feb 27;279(9):8212-8. DOI:10.1074/jbc.M312230200 |
- Erb L, Liu J, Ockerhausen J, Kong Q, Garrad RC, Griffin K, Neal C, Krugh B, Santiago-Pérez LI, González FA, Gresham HD, Turner JT, and Weisman GA. An RGD sequence in the P2Y(2) receptor interacts with alpha(V)beta(3) integrins and is required for G(o)-mediated signal transduction. J Cell Biol. 2001 Apr 30;153(3):491-501. DOI:10.1083/jcb.153.3.491 |
