User:Korey Griffin
From OpenWetWare
Contact
- Korey Griffin MS
- Santa Cruz Biotechnology, Inc.
- Email me through OpenWetWare
Bench research leads to advancements in knowledge & health. Confidence in reagent quality & protocol is essential for success in molecular & cell biology. There is ongoing need to facilitate scientists in creating successful R&D efforts in every phase, through communication on reagent quality & procedure optimization.
Hundreds of variables within molecular/cell protocols determine reliable data. Apparent & straight-forward experiments (ie immuno (western) blotting) contain many important stages for consideration. Presenting below, a valuable collection of resources, that will improve awareness & success.
Methods & Techniques
- Antibody Basics
- Antibody Peptide Neutralization
- Antibody Protein Coupling (F:P) Ratios
- Antibody Elution Buffers
- Antibody Related Solutions & Recipes
- Antigen Retrieval Technique
- Chromatin Immunoprecipitation
- Conditioned Medium Preparation (Growth Factor/Cytokine)
- Dual Immunofluorescence Cell Staining
- Flow Cytometry prep & labeling
- Immunoprecipitation Guide
- Immunohistochemistry Paraffin
- Immunofluorescence Cell Staining
- In Vitro Kinase Assay
- Isolate Leukocytes from whole blood
- Lentivirus Technology
- Membrane Stripping and Reprobing
- Nested RT-PCR
- Nuclear Extract Preparation
- Puromycin Selection for shRNA
- shRNA Transfection
- siRNA Transfection
- Sodium Orthovanadate Activation
- Western Blotting Guide
- Western Blot Optimization
- Zinc Fixation
Links
- ATCC Cell Lines
- Organelle and Lineage Markers
- DOT guidelines
- Fluorochrome Table
- Human Protein Atlas
- Oligo Tm
