User:Kristina M Holton/Ovarian Notebook 2007 9 26

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Ambion RecoverAll Protocol: 12 samples

Notes on Protocol:

  • Deparaffinization Step 2: Sample 3: Red; Sample 9: Brown
  • Deparaffinization Step 3: Sample 11: small pellet; Sample 12: small pellet
  • Nucleic Acid Isolation Step 2: Most not fully digested; Sample 5: Light Green; Sample 7: Green
Sample
Sample ID
ng/ul
260/280
260/230
Vol
Total
Dilution
Total to Illumina
1
502-35932-D
95.01
1.97
.85
60ul
5.7ug
none
2.7ug
2
502-35973-G
31.08
2.01
.48
60ul
1.9ug
none
1.9ug
3
502-36090-J
19.43
1.90
.20
60ul
1.2ug
none
1.2ug
4
502-36200-M
124.29
2.00
.94
60ul
7.4ug
30ul + 7.2ul H20
3.7ug
5
502-36433-AA
405.71
1.90
1.18
60ul
24ug
30ul + 92ul H20
12ug
6
502-36752-E
312.07
2.04
1.76
60ul
19ug
30ul + 64ul H20
9.5ug
7
502-36839-I
95.53
2.05
.27
60ul
5.7ug
none
2.8ug
8
502-36878-B
462.47
2.04
1.07
60ul
28ug
30ul + 101ul H20
14ug
9
502-36925-B
327.75
2.03
.87
60ul
20ug
30ul + 68ul H20
10ug
10
502-37203-AE
172.73
1.96
.88
60ul
10ug
30ul + 22ul H20
5ug
11
599-35521-E
32.59
1.87
.61
60ul
2ug
none
2ug
12
599-35521-N
131.92
1.93
.86
60ul
8ug
30ul + 9ul H20
4ug
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