User:Lance Martin
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Electronic memory & logic devices
Devices
- Transistors
- Logic gates
- Latches
- D-type flip-flops
- JK flip-flops
Summary presentation
Media:Electronic_Memory_&_Logic_Devices.ppt
Electronic counters & system architecture
Architectures
- Cascade
- Asynchronous
- Synchronous
Summary presentation
Native biological memory & logic
Basic requirements
- Reliably holds state
- Controllable state change
- Many additional application-specific requirements
Systems
- Recombination
- Types of enzymes
- Kinetic modeling recombinase mechanism
- DNA methylation
- Others?
- Feedback loops
- Bi-stable networks
Design of engineered biological memory & logic
Basic construction / design principles
- Summary of reviews by
- Voight
- Endy
- Arkin
Computational modeling
- Review of
- Collins toggle switch
- Elowitz repressilator
Engineered biological memory & logic
Of particular interest to us
- Ham & Arkin inversion switch
- Harvard/BU 2004 iGem Int/Xis inversion switch
- Their final presentation
- My notes on this work
- DNA methylation switch
My projects
Gemini
- Summary
- synBERC poster
- Current focus
- What is the unique application for compact (LacZa-GFP) dual reporter?
- ��What is the dynamic range (transfer function) for the LacZa-GFP fusion construct?�
- Sequence: understand what we have�.
- Determine method to modulate PoPS input �(use the existing, different promoters or build with inducible promoter).
- Set up assays �(plate reader for GFP and beta-gal)��
- How does this compare to full length LacZ-GFP fusion, GFP, and LacZ?�
- With information from the above in hand, determine additional work necessary to make genetically identical constructs (same promoter, RBS, reporters – from Meagan)
